Pregnancy by assisted reproductive technology is associated with shorter telomere length in neonates

Toshiko Minamoto, Kentaro Nakayama, Tomoka Ishibashi, Masako Ishikawa, Kohei Nakamura, Hitomi Yamashita, Kamrunnahar Shanta, Hossain Mohammad Mahmud, Sultana Razia, Kouji Iida, Gyosuke Sakashita, Tsukasa Nakamura, Hideyuki Kanda, Satoru Kyo

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Telomere length (TL) influences the development of lifestyle-related diseases, and neonatal TL may influence their prevalence. Various factors have been reported to affect neonatal TL. Although the fetus is exposed to multiple conditions in utero, the main factors affecting the shortening of neonatal TL are still not known. In this study, we sought to identify factors that influence fetal TL. A total of 578 mother-newborn pairs were included for TL analysis. TL was measured in genomic DNA extracted from cord blood samples using quantitative PCR. The clinical factors examined at enrollment included the following intrauterine environmental factors: maternal age, assisted reproductive technology (ART) used, body mass index (BMI), gestational diabetes mellitus (GDM), maternal stress, smoking, alcohol consumption, preterm delivery, small-for-gestational-age, neonatal sex, and placental weight. Univariate and multivariate regression analyses were used to verify the relationship between neonatal TL and these clinical factors. The median neonatal TL to single-copy gene ratio was 1.0. Pregnancy with ART was among the 11 factors associated with shorter neonatal TL. From multiple regression analysis, we determined that neonatal TL was significantly shorter for pregnancies in the ART group than in the other groups. We conclude that pregnancy with ART is associated with shorter neonatal TL.

Original languageEnglish
Article number9688
Pages (from-to)1-10
Number of pages10
JournalInternational journal of molecular sciences
Issue number24
Publication statusPublished - Dec 2 2020


  • Assisted reproductive technology
  • Developmental origins of health and disease
  • Lifestyle
  • Neonates
  • Pregnancy
  • Telomere length

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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