Preparation and antioxidant activity of enzymatic hydrolysates from purple sea urchin (Strongylocentrotus nudus) gonad

Lei Qin, Bei Wei Zhu, Da Yong Zhou, Hai Tao Wu, Hui Tan, Jing Feng Yang, Dong Mei Li, Xiu Ping Dong, Yoshiyuki Murata

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)


Purple sea urchin (Strongylocentrotus nudus) gonad was treated separately with neutral protease, papain, pepsin and trypsin. The resultant hydrolysates were fractionated using a series of ultrafiltration membranes (molecular weight cut-offs of 10, 5, 3 and 1 kDa). Five fractions were prepared from each hydrolysate and the corresponding molecular weight ranges were below 10 kDa, 5-10 kDa, 3-5 kDa, 1-3 kDa and below 1 kDa. The peptide fractions were evaluated for antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and reducing power assay. Results indicated that all peptide fractions possessed DPPH radical scavenging capacity and reducing power in a dose-dependent manner. For all four hydrolysates, the below 1 kDa fractions exhibited the highest DPPH radical scavenging capacity. The below 1 kDa fractions prepared with neutral protease, papain and pepsin, and the 1-3 kDa fraction prepared with trypsin showed the highest reducing capacity among corresponding hydrolysates.

Original languageEnglish
Pages (from-to)1113-1118
Number of pages6
JournalLWT - Food Science and Technology
Issue number4
Publication statusPublished - May 1 2011


  • Antioxidant activity
  • Hydrolysates
  • Peptides
  • Sea urchin (Strongylocentrotus nudus)
  • Ultrafiltration

ASJC Scopus subject areas

  • Food Science


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