TY - JOUR
T1 - Preparation and antioxidant activity of enzymatic hydrolysates from purple sea urchin (Strongylocentrotus nudus) gonad
AU - Qin, Lei
AU - Zhu, Bei Wei
AU - Zhou, Da Yong
AU - Wu, Hai Tao
AU - Tan, Hui
AU - Yang, Jing Feng
AU - Li, Dong Mei
AU - Dong, Xiu Ping
AU - Murata, Yoshiyuki
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Purple sea urchin (Strongylocentrotus nudus) gonad was treated separately with neutral protease, papain, pepsin and trypsin. The resultant hydrolysates were fractionated using a series of ultrafiltration membranes (molecular weight cut-offs of 10, 5, 3 and 1 kDa). Five fractions were prepared from each hydrolysate and the corresponding molecular weight ranges were below 10 kDa, 5-10 kDa, 3-5 kDa, 1-3 kDa and below 1 kDa. The peptide fractions were evaluated for antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and reducing power assay. Results indicated that all peptide fractions possessed DPPH radical scavenging capacity and reducing power in a dose-dependent manner. For all four hydrolysates, the below 1 kDa fractions exhibited the highest DPPH radical scavenging capacity. The below 1 kDa fractions prepared with neutral protease, papain and pepsin, and the 1-3 kDa fraction prepared with trypsin showed the highest reducing capacity among corresponding hydrolysates.
AB - Purple sea urchin (Strongylocentrotus nudus) gonad was treated separately with neutral protease, papain, pepsin and trypsin. The resultant hydrolysates were fractionated using a series of ultrafiltration membranes (molecular weight cut-offs of 10, 5, 3 and 1 kDa). Five fractions were prepared from each hydrolysate and the corresponding molecular weight ranges were below 10 kDa, 5-10 kDa, 3-5 kDa, 1-3 kDa and below 1 kDa. The peptide fractions were evaluated for antioxidant activity by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and reducing power assay. Results indicated that all peptide fractions possessed DPPH radical scavenging capacity and reducing power in a dose-dependent manner. For all four hydrolysates, the below 1 kDa fractions exhibited the highest DPPH radical scavenging capacity. The below 1 kDa fractions prepared with neutral protease, papain and pepsin, and the 1-3 kDa fraction prepared with trypsin showed the highest reducing capacity among corresponding hydrolysates.
KW - Antioxidant activity
KW - Hydrolysates
KW - Peptides
KW - Sea urchin (Strongylocentrotus nudus)
KW - Ultrafiltration
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U2 - 10.1016/j.lwt.2010.10.013
DO - 10.1016/j.lwt.2010.10.013
M3 - Article
AN - SCOPUS:78751702460
SN - 0023-6438
VL - 44
SP - 1113
EP - 1118
JO - LWT - Food Science and Technology
JF - LWT - Food Science and Technology
IS - 4
ER -