Preparation and characterization of recombinant golgin tethers

Ayano Satoh, Matthew Beard, Graham Warren

Research output: Contribution to journalReview articlepeer-review

7 Citations (Scopus)


Golgin tethers are integral or peripheral Golgi proteins with predicted coiled-coil domains and many are known to interact directly with small GTPases of the Ypt/Rab or Arl families. Here we describe the preparation of recombinant golgins: GM130, p115 (and truncations thereof), the N-terminal fragment of giantin, CASP, and golgin-84.

Original languageEnglish
Article number26
Pages (from-to)279-296
Number of pages18
JournalMethods in Enzymology
Publication statusPublished - 2005
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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