TY - JOUR
T1 - Preparation of DNA containing 7-methylguanine as unique lesions.
AU - Asagoshi, K.
AU - Terato, H.
AU - Ohyama, Y.
AU - Ide, H.
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 1999
Y1 - 1999
N2 - The predominant adduct produced by both endogenous and exogenous methylating agents is 7-methylguanine(m7G). Most studies on the repair of m7G reported so far used methylated DNA as substrates which contained other unintended lesions. In the presented study, DNA substrates containing m7G as unique lesions were prepared by DNA polymerase reactions. Using these substrates, damage recognition of E. coli 3-methyladenine DNA glycosylase II (AlkA) was analyzed. The obtained results suggested that the repair rate of m7G by AlkA was affected by the flanking sequence context of the lesion.
AB - The predominant adduct produced by both endogenous and exogenous methylating agents is 7-methylguanine(m7G). Most studies on the repair of m7G reported so far used methylated DNA as substrates which contained other unintended lesions. In the presented study, DNA substrates containing m7G as unique lesions were prepared by DNA polymerase reactions. Using these substrates, damage recognition of E. coli 3-methyladenine DNA glycosylase II (AlkA) was analyzed. The obtained results suggested that the repair rate of m7G by AlkA was affected by the flanking sequence context of the lesion.
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U2 - 10.1093/nass/42.1.83
DO - 10.1093/nass/42.1.83
M3 - Article
C2 - 10780390
AN - SCOPUS:0033289081
SN - 0261-3166
SP - 83
EP - 84
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
IS - 42
ER -