Abstract
We have performed the development of nano-inorgenic particle/polymer/DNA composites by ultra high pressure technology for gene transfection. Nano scaled hydroxy apatite (HAp) was used as nano-inorgenic particle. Polyvinyl alcohol (PVA, 5, 7.5, 10w/v%) aqueous solution mixed with HAp(0.01-10mg/ml) and DNA, then treated by ultra high pressure at 10,000 atm for 10min. HAp/PVA/DNA hydrogel was obtained. By SEM observation, HAp particles on the surface of the hydrogel were observed. By DNA staining, the including of DNA in the hydrogel was confirmed. In order to investigate the cell affinity of the hydrogel, various cell lines were added on the hydrogels. In the case of PVA/DNA hydrogel, almost all of cells were not adhered. On the other hand, the effective cell adhesion was observed on HAp/PVA/DNA hydrogel.
| Original language | English |
|---|---|
| Number of pages | 1 |
| Publication status | Published - Oct 19 2006 |
| Externally published | Yes |
| Event | 55th SPSJ Annual Meeting - Nagoya, Japan Duration: May 24 2006 → May 26 2006 |
Other
| Other | 55th SPSJ Annual Meeting |
|---|---|
| Country/Territory | Japan |
| City | Nagoya |
| Period | 5/24/06 → 5/26/06 |
Keywords
- Hydrogel
- Hydroxy apatite
- Transfection
- Ultra high pressure treatment
ASJC Scopus subject areas
- Engineering(all)