Priming of neutrophil oxidative burst in diabetes requires preassembly of the NADPH oxidase

Kazuhiro Omori, Taisuke Ohira, Yushi Uchida, Srinivas Ayilavarapu, Eraldo L. Batista, Motohiko Yagi, Tomoyuki Iwata, Hongsheng Liu, Hatice Hasturk, Alpdogan Kantarci, Thomas E. Van Dyke

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)


Hyperglycemia associated with diabetes mellitus results in the priming of neutrophils leading to oxidative stress that is, in part, responsible for diabetic complications. p47phox, a NADPH oxidase cytosolic subunit, is a key protein in the assembly of the NADPH oxidase leading to superoxide generation. Little is known about the priming mechanism of oxidative pathways in neutrophils of people with diabetes. In this study, the kinetics of p47 phox activation was investigated by comparing neutrophils from diabetic and healthy subjects, and the mechanism of hyperglycemia-induced changes was studied by using neutrophil-like HL-60 cells as a model. In resting neutrophils from diabetic subjects, p47phox prematurely translocates to the cell membrane and preassembles with p22phox, a NADPH oxidase membrane subunit. This premature p47phox translocation and preassembly with p22phox were also observed in HL-60 cells cultured with high glucose (HG; 25 mM) and with the specific ligand for the receptor for advanced glycation end products (RAGE), S100B. Phosphorylation of ERK1/2, but not p38 MAPK, was the primary signaling pathway, as evidenced by PD98059 suppressing the translocation of p47phox in HL-60 cells incubated with HG and S100B. HL-60 cells cultured in HG and S100B exhibited a 1.8-fold increase in fMLP-induced superoxide generation compared with those cultured in normal glucose (5.5 mM). These data suggest that HG and increased AGE prime neutrophils and increase oxidative stress inducing the translocation of p47 phox to the cell membrane and preassembly with p22phox by stimulating a RAGE-ERK1/2 pathway.

Original languageEnglish
Pages (from-to)292-301
Number of pages10
JournalJournal of Leukocyte Biology
Issue number1
Publication statusPublished - Jul 1 2008
Externally publishedYes


  • Cell activation
  • Inflammation
  • Signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology


Dive into the research topics of 'Priming of neutrophil oxidative burst in diabetes requires preassembly of the NADPH oxidase'. Together they form a unique fingerprint.

Cite this