Production and characterization of a complete set of wheat-wild barley (Hordeum vulgare ssp. spontaneum) chromosome addition lines

A wild barley, Hordeum vulgare L. ssp. spontaneum (C. Koch.) Thell. (syn. H. spontaneum C. Koch.) is considered to be the progenitor of cultivated barley (H. vulgare L. ssp. vulgare). A complete set of Triticum aestivum L. cv. 'Shinchunaga'-H. spontaneum OUH602 whole chromosome addition lines, and 5 telosomic addition lines were developed. Chromosome constitutions of the addition lines were confirmed by C-banding and genomic in situ hybridization. Addition lines for the entire 1H chromosome and its long arm are only available as monosomic and monotelosomic additions, respectively, because of sterility. All other lines were recovered as disomic or ditelosomic additions. Self-sterile monosomic 1H and monotelosomic 1HL addition lines can be reproduced by backcrossing the plants having simultaneous addition of the long arm of barley chromosome 6H, because the plants with such chromosome constitutions recover partial female fertility. In general, the phenotypic alternations observed in each of the wheat-H. spontaneum OUH602 addition lines were similar to those previously noted in the corresponding lines of the 'Chinese Spring' wheat-'Betzes' barley addition series and the primary trisomic series of the H. spontaneum accession OUH602. Monotelosomic 1HL addition and ditelosomic 1HS addition are especially valuable for unequivocal chromosome arm localization of barley genes and markers clustered around the centromeric region of 1H. The utility of the wheat-H. spontaneum addition lines is discussed.