Production of two phosphoproteins from the NS5A region of the hepatitis C viral genome

Takashi Kaneko, Yasunori Tanji, Shinya Satoh, Makolo Hijikata, Shinichi Asabe, Koichi Kumura, Kunitada Shimotohno

Research output: Contribution to journalArticlepeer-review

192 Citations (Scopus)


Hepatitis C virus produces about 12 viral proteins by proteolytic cleavage of the viral polyprotein precursor produced from the largest open reading frame in the viral genome. We have analyzed the production of viral nonstructural proteins with in vivo transient expression system using COS-1 cells. Two proteins, a 56-kDa protein and a 58-kDa protein, were produced from the nonstructural region 5A (NS5A), which has the potential to produce a 49 kDa protein. We showed that these proteins are phosphorylated at the serine residues. The presence of the two proteins was reflected by different degrees of phosphorylation. Moreover, the hyper-phosphorylation of p58 was shown to depend on the presence of NS4A, another hepatitis C virus protein.

Original languageEnglish
Pages (from-to)320-326
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number1
Publication statusPublished - Nov 30 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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