TY - JOUR
T1 - Prolonged survival of donor-specific rat intestinal allograft by administration of bone-marrow-derived immature dendritic cells
AU - Sheng Sun, Dong
AU - Iwagaki, Hiromi
AU - Ozaki, Michitaka
AU - Ogino, Tetsuya
AU - Kusaka, Satoshi
AU - Fujimoto, Yoshimi
AU - Murata, Hiroshi
AU - Sadamori, Hiroshi
AU - Matsukawa, Hiroyoshi
AU - Tanaka, Noriaki
AU - Yagi, Takahito
PY - 2005/3
Y1 - 2005/3
N2 - It has been reported that intraportal administration of donor antigens induced donor-specific hyporesponsiveness. We studied here the effects of transplantation of BM-derived immature dendritic cells (imDCs) and mature DCs (mDCs) via portal vein on rat small intestinal allograft survival. This study comprised four treatment groups: 1) untreated controls; 2) FK506 alone; 3) intraportal donor-specific BM-derived imDCs transplantation+FK506; 4) mDCs/Tx+FK506. Allograft survival was minimal in control group (5.2±0.8 days) and maximal in imDC+FK506 group (28.4±3.0 days). The rats in mDC+FK506 group showed systemic inflammatory reaction due to GVHR, and died within 10 days after transplantation. The in vitro MLR reaction using imDCs was also strongly inhibited both in direct and indirect recognition pathways. The impact of imDCs for the specific induction of transplant tolerance may suggest that immunization with donor-specific imDCs has therapeutic potential in organ transplantation.
AB - It has been reported that intraportal administration of donor antigens induced donor-specific hyporesponsiveness. We studied here the effects of transplantation of BM-derived immature dendritic cells (imDCs) and mature DCs (mDCs) via portal vein on rat small intestinal allograft survival. This study comprised four treatment groups: 1) untreated controls; 2) FK506 alone; 3) intraportal donor-specific BM-derived imDCs transplantation+FK506; 4) mDCs/Tx+FK506. Allograft survival was minimal in control group (5.2±0.8 days) and maximal in imDC+FK506 group (28.4±3.0 days). The rats in mDC+FK506 group showed systemic inflammatory reaction due to GVHR, and died within 10 days after transplantation. The in vitro MLR reaction using imDCs was also strongly inhibited both in direct and indirect recognition pathways. The impact of imDCs for the specific induction of transplant tolerance may suggest that immunization with donor-specific imDCs has therapeutic potential in organ transplantation.
KW - Dendritic cells
KW - Small intestinal transplantation
KW - Tolerance
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U2 - 10.1016/j.trim.2004.12.002
DO - 10.1016/j.trim.2004.12.002
M3 - Article
C2 - 15814277
AN - SCOPUS:20144389764
SN - 0966-3274
VL - 14
SP - 17
EP - 20
JO - Transplant Immunology
JF - Transplant Immunology
IS - 1
ER -