Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)

K. Ochiai; H. Oda; S. Shono; Y. Kato; S. Sugihara; S. Nakazawa; D. Azakami; M. Michishita; E. Onozawa; M. Bonkobara; T. Sako; L. Shun-Ai; H. Ueki; M. Watanabe; T. Omi

doi:10.1111/vco.12254

Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)

K. Ochiai, H. Oda, S. Shono, Y. Kato, S. Sugihara, S. Nakazawa, D. Azakami, M. Michishita, E. Onozawa, M. Bonkobara, T. Sako, L. Shun-Ai, H. Ueki, M. Watanabe, T. Omi

University Hospital

Research output: Contribution to journal › Article › peer-review

Abstract

Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.

Original language	English
Pages (from-to)	1181-1186
Number of pages	6
Journal	Veterinary and Comparative Oncology
Volume	15
Issue number	4
DOIs	https://doi.org/10.1111/vco.12254
Publication status	Published - Dec 2017

Keywords

REIC/Dkk-3
cat
feline
mammary gland tumours
tumour suppressor gene

ASJC Scopus subject areas

veterinary(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/vco.12254

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Ochiai, K., Oda, H., Shono, S., Kato, Y., Sugihara, S., Nakazawa, S., Azakami, D., Michishita, M., Onozawa, E., Bonkobara, M., Sako, T., Shun-Ai, L., Ueki, H., Watanabe, M., & Omi, T. (2017). Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3). Veterinary and Comparative Oncology, 15(4), 1181-1186. https://doi.org/10.1111/vco.12254

Ochiai, K, Oda, H, Shono, S, Kato, Y, Sugihara, S, Nakazawa, S, Azakami, D, Michishita, M, Onozawa, E, Bonkobara, M, Sako, T, Shun-Ai, L, Ueki, H, Watanabe, M & Omi, T 2017, 'Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)', Veterinary and Comparative Oncology, vol. 15, no. 4, pp. 1181-1186. https://doi.org/10.1111/vco.12254

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title = "Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)",

abstract = "Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.",

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author = "K. Ochiai and H. Oda and S. Shono and Y. Kato and S. Sugihara and S. Nakazawa and D. Azakami and M. Michishita and E. Onozawa and M. Bonkobara and T. Sako and L. Shun-Ai and H. Ueki and M. Watanabe and T. Omi",

note = "Funding Information: This work was supported by KAKENHI scientific research grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 15 K07754). Publisher Copyright: {\textcopyright} 2016 John Wiley & Sons Ltd",

year = "2017",

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doi = "10.1111/vco.12254",

language = "English",

volume = "15",

pages = "1181--1186",

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AU - Ochiai, K.

AU - Oda, H.

AU - Shono, S.

AU - Kato, Y.

AU - Sugihara, S.

AU - Nakazawa, S.

AU - Azakami, D.

AU - Michishita, M.

AU - Onozawa, E.

AU - Bonkobara, M.

AU - Sako, T.

AU - Shun-Ai, L.

AU - Ueki, H.

AU - Watanabe, M.

AU - Omi, T.

N1 - Funding Information: This work was supported by KAKENHI scientific research grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 15 K07754). Publisher Copyright: © 2016 John Wiley & Sons Ltd

PY - 2017/12

Y1 - 2017/12

N2 - Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.

AB - Reduced expression in immortalized cells (REIC/Dkk-3), a member of the human Dickkopf (Dkk) family, is a growth suppressor in human and canine mammary tumours. Mammary gland tumours are common neoplasms with high malignancy in female cats. The purpose of this study was to clone the feline REIC/Dkk-3 homolog, investigate its expression in cell lines established from feline mammary gland tumours, and test its tumour suppressor function. Western blot analysis revealed that expression of the REIC/Dkk-3 protein was reduced in feline mammary carcinoma cell lines. Forced expression of REIC/Dkk-3 induced apoptosis in feline mammary tumour cell lines. These results demonstrate that REIC/Dkk-3 expression, which is downregulated in feline mammary tumour cell lines, results in the induction of apoptosis in these cells. Our findings suggest that feline REIC/Dkk-3 represents a potential molecular target for the development of therapies against feline mammary cancers.

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KW - cat

KW - feline

KW - mammary gland tumours

KW - tumour suppressor gene

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Properties of the feline tumour suppressor reduced expression in immortalized cells (REIC/Dkk-3)

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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