Propofol suppresses a hyperpolarization-activated inward current in rat hippocampal CA1 neurons

Makoto Funahashi; Hitoshi Higuchi; Takuya Miyawaki; Masahiko Shimada; Ryuji Matsuo

doi:10.1016/S0304-3940(01)02169-3

Propofol suppresses a hyperpolarization-activated inward current in rat hippocampal CA1 neurons

Makoto Funahashi, Hitoshi Higuchi, Takuya Miyawaki, Masahiko Shimada, Ryuji Matsuo

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

We examined the effect of propofol and thiopental, intravenous anesthetics, on the hyperpolarization-activated inward current (I_H), whose functional role on the neuronal activity has been evaluated. Whole-cell recordings of I_H evoked by hyperpolarizing step pulses were taken from hippocampal CA1 neurons in rat brain slices. Propofol reduced I_H current in a dose-dependent manner. However, thiopental had no significant effect on the activation of I_H. According to the functional role of I_H, the suppression of I_H should result in a reduction of neuronal activity. We suggest that the effectiveness of propofol as an anticonvulsant or an antiemetic is associated with the blockade of the I_H channel.

Original language	English
Pages (from-to)	177-180
Number of pages	4
Journal	Neuroscience Letters
Volume	311
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(01)02169-3
Publication status	Published - Oct 5 2001

Keywords

Brain slices
CA1
Hyperpolarization-activated inward current
Propofol
Rat
Thiopental
Whole-cell patch-clamp

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1016/S0304-3940(01)02169-3

Cite this

@article{f53fd68846274ad48d008f545b7f71ed,

title = "Propofol suppresses a hyperpolarization-activated inward current in rat hippocampal CA1 neurons",

abstract = "We examined the effect of propofol and thiopental, intravenous anesthetics, on the hyperpolarization-activated inward current (IH), whose functional role on the neuronal activity has been evaluated. Whole-cell recordings of IH evoked by hyperpolarizing step pulses were taken from hippocampal CA1 neurons in rat brain slices. Propofol reduced IH current in a dose-dependent manner. However, thiopental had no significant effect on the activation of IH. According to the functional role of IH, the suppression of IH should result in a reduction of neuronal activity. We suggest that the effectiveness of propofol as an anticonvulsant or an antiemetic is associated with the blockade of the IH channel.",

keywords = "Brain slices, CA1, Hyperpolarization-activated inward current, Propofol, Rat, Thiopental, Whole-cell patch-clamp",

author = "Makoto Funahashi and Hitoshi Higuchi and Takuya Miyawaki and Masahiko Shimada and Ryuji Matsuo",

note = "Funding Information: This research was supported by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan, Kato Memorial Foundation and Kobayashi Magobei Memorial Foundation.",

year = "2001",

month = oct,

day = "5",

doi = "10.1016/S0304-3940(01)02169-3",

language = "English",

volume = "311",

pages = "177--180",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Propofol suppresses a hyperpolarization-activated inward current in rat hippocampal CA1 neurons

AU - Funahashi, Makoto

AU - Higuchi, Hitoshi

AU - Miyawaki, Takuya

AU - Shimada, Masahiko

AU - Matsuo, Ryuji

N1 - Funding Information: This research was supported by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan, Kato Memorial Foundation and Kobayashi Magobei Memorial Foundation.

PY - 2001/10/5

Y1 - 2001/10/5

N2 - We examined the effect of propofol and thiopental, intravenous anesthetics, on the hyperpolarization-activated inward current (IH), whose functional role on the neuronal activity has been evaluated. Whole-cell recordings of IH evoked by hyperpolarizing step pulses were taken from hippocampal CA1 neurons in rat brain slices. Propofol reduced IH current in a dose-dependent manner. However, thiopental had no significant effect on the activation of IH. According to the functional role of IH, the suppression of IH should result in a reduction of neuronal activity. We suggest that the effectiveness of propofol as an anticonvulsant or an antiemetic is associated with the blockade of the IH channel.

AB - We examined the effect of propofol and thiopental, intravenous anesthetics, on the hyperpolarization-activated inward current (IH), whose functional role on the neuronal activity has been evaluated. Whole-cell recordings of IH evoked by hyperpolarizing step pulses were taken from hippocampal CA1 neurons in rat brain slices. Propofol reduced IH current in a dose-dependent manner. However, thiopental had no significant effect on the activation of IH. According to the functional role of IH, the suppression of IH should result in a reduction of neuronal activity. We suggest that the effectiveness of propofol as an anticonvulsant or an antiemetic is associated with the blockade of the IH channel.

KW - Brain slices

KW - CA1

KW - Hyperpolarization-activated inward current

KW - Propofol

KW - Rat

KW - Thiopental

KW - Whole-cell patch-clamp

UR - http://www.scopus.com/inward/record.url?scp=0035812868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035812868&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(01)02169-3

DO - 10.1016/S0304-3940(01)02169-3

M3 - Article

C2 - 11578823

AN - SCOPUS:0035812868

SN - 0304-3940

VL - 311

SP - 177

EP - 180

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 3

ER -

Propofol suppresses a hyperpolarization-activated inward current in rat hippocampal CA1 neurons

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this