Protection Against Ischemic Brain Damage in Rats by Immunophilin Ligand GPI-1046

Feng Li; Nobuhiko Omori; Takeshi Hayashi; Guang Jin; Keiko Sato; Isao Nagano; Mikio Shoji; Koji Abe

doi:10.1002/jnr.20067

Protection Against Ischemic Brain Damage in Rats by Immunophilin Ligand GPI-1046

Feng Li, Nobuhiko Omori, Takeshi Hayashi, Guang Jin, Keiko Sato, Isao Nagano, Mikio Shoji, Koji Abe

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

To determine the effect of immunophilin ligand GPI-1046 on ischemic brain injury, 90 min of transient middle cerebral artery occlusion (MCAO) was carried out in rat brains. In contrast to cases treated with vehicle, the infarct volume was reduced greatly and rotamase activity was inhibited significantly at 24 hr of reperfusion by treatment with GPI-1046. Immunoreactivity and the number of cells stained positively for FKBP12, FKBP52, caspase-8, cytochrome c, and caspase-3 were also reduced markedly in the brain after GPI-1046 treatment. The present results suggest that GPI-1046 significantly decreased infarct volume and provided neuroprotective effect on rats after transient focal cerebral ischemia by inhibiting the increase of rotamase activity and of the number of FKBP12-, FKBP52-, caspase-8-, cytochrome c-, and caspase-3-positive cells in the ischemic area.

Original language	English
Pages (from-to)	383-389
Number of pages	7
Journal	Journal of Neuroscience Research
Volume	76
Issue number	3
DOIs	https://doi.org/10.1002/jnr.20067
Publication status	Published - May 1 2004
Externally published	Yes

Keywords

Caspase
FKBPs
GPI-1046
Rotamase

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

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10.1002/jnr.20067

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title = "Protection Against Ischemic Brain Damage in Rats by Immunophilin Ligand GPI-1046",

abstract = "To determine the effect of immunophilin ligand GPI-1046 on ischemic brain injury, 90 min of transient middle cerebral artery occlusion (MCAO) was carried out in rat brains. In contrast to cases treated with vehicle, the infarct volume was reduced greatly and rotamase activity was inhibited significantly at 24 hr of reperfusion by treatment with GPI-1046. Immunoreactivity and the number of cells stained positively for FKBP12, FKBP52, caspase-8, cytochrome c, and caspase-3 were also reduced markedly in the brain after GPI-1046 treatment. The present results suggest that GPI-1046 significantly decreased infarct volume and provided neuroprotective effect on rats after transient focal cerebral ischemia by inhibiting the increase of rotamase activity and of the number of FKBP12-, FKBP52-, caspase-8-, cytochrome c-, and caspase-3-positive cells in the ischemic area.",

keywords = "Caspase, FKBPs, GPI-1046, Rotamase",

author = "Feng Li and Nobuhiko Omori and Takeshi Hayashi and Guang Jin and Keiko Sato and Isao Nagano and Mikio Shoji and Koji Abe",

year = "2004",

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T1 - Protection Against Ischemic Brain Damage in Rats by Immunophilin Ligand GPI-1046

AU - Li, Feng

AU - Omori, Nobuhiko

AU - Hayashi, Takeshi

AU - Jin, Guang

AU - Sato, Keiko

AU - Nagano, Isao

AU - Shoji, Mikio

AU - Abe, Koji

PY - 2004/5/1

Y1 - 2004/5/1

N2 - To determine the effect of immunophilin ligand GPI-1046 on ischemic brain injury, 90 min of transient middle cerebral artery occlusion (MCAO) was carried out in rat brains. In contrast to cases treated with vehicle, the infarct volume was reduced greatly and rotamase activity was inhibited significantly at 24 hr of reperfusion by treatment with GPI-1046. Immunoreactivity and the number of cells stained positively for FKBP12, FKBP52, caspase-8, cytochrome c, and caspase-3 were also reduced markedly in the brain after GPI-1046 treatment. The present results suggest that GPI-1046 significantly decreased infarct volume and provided neuroprotective effect on rats after transient focal cerebral ischemia by inhibiting the increase of rotamase activity and of the number of FKBP12-, FKBP52-, caspase-8-, cytochrome c-, and caspase-3-positive cells in the ischemic area.

AB - To determine the effect of immunophilin ligand GPI-1046 on ischemic brain injury, 90 min of transient middle cerebral artery occlusion (MCAO) was carried out in rat brains. In contrast to cases treated with vehicle, the infarct volume was reduced greatly and rotamase activity was inhibited significantly at 24 hr of reperfusion by treatment with GPI-1046. Immunoreactivity and the number of cells stained positively for FKBP12, FKBP52, caspase-8, cytochrome c, and caspase-3 were also reduced markedly in the brain after GPI-1046 treatment. The present results suggest that GPI-1046 significantly decreased infarct volume and provided neuroprotective effect on rats after transient focal cerebral ischemia by inhibiting the increase of rotamase activity and of the number of FKBP12-, FKBP52-, caspase-8-, cytochrome c-, and caspase-3-positive cells in the ischemic area.

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KW - FKBPs

KW - GPI-1046

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Protection Against Ischemic Brain Damage in Rats by Immunophilin Ligand GPI-1046

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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