Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats

Takehit Suzuki, Hideo Fukuoka, Setsuo Ushikoshi, Reiichiro Sato, Hidetoshi Morita, Tatsuya Takizawa

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4 Citations (Scopus)


Hepatoprotective effects of Rhizopusoryzae/U-1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl4)-induced liver-injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl4 (125mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl4. (ii) RU pretreatment (100mg/kg) increased 5-bromo-2′-deoxyuridine incorporation at 48h after CCl4 treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl4 administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin-like growth factor-I (IGF-I) gene expression in hepatocytes injured by CCl4 treatment. From these results, we conclude that oral pre-administration of RU was effective to suppress liver injury induced by the subsequent oral CCl4 administration, and RU-induced increase in IGF-I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.

Original languageEnglish
Pages (from-to)532-540
Number of pages9
JournalAnimal Science Journal
Issue number5
Publication statusPublished - May 1 2015
Externally publishedYes


  • Insulin-like growth factor 1 (IGF-1)
  • Liver injury
  • Rhizopus oryzae

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences


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