Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

Naoya Ohara; Masanori Matsuoka; Hiroko Nomaguchi; Mariko Naito; Takeshi Yamada

doi:10.1016/S0264-410X(00)00439-4

Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

Naoya Ohara, Masanori Matsuoka, Hiroko Nomaguchi, Mariko Naito, Takeshi Yamada

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

Original language	English
Pages (from-to)	1906-1910
Number of pages	5
Journal	Vaccine
Volume	19
Issue number	15-16
DOIs	https://doi.org/10.1016/S0264-410X(00)00439-4
Publication status	Published - Feb 28 2001
Externally published	Yes

Keywords

Ag85
BCG
Leprosy
Recombinant
Vaccine

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(00)00439-4

Cite this

Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates. / Ohara, Naoya; Matsuoka, Masanori; Nomaguchi, Hiroko et al.
In: Vaccine, Vol. 19, No. 15-16, 28.02.2001, p. 1906-1910.

Research output: Contribution to journal › Article › peer-review

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title = "Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Gu{\'e}rin over-producing three putative protective antigen candidates",

abstract = "The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Gu{\'e}rin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.",

keywords = "Ag85, BCG, Leprosy, Recombinant, Vaccine",

author = "Naoya Ohara and Masanori Matsuoka and Hiroko Nomaguchi and Mariko Naito and Takeshi Yamada",

note = "Funding Information: This work was partly supported by grants from the U.S.–Japan Cooperative Medical Science Program and by a {\textquoteleft}Research Grant on Emerging and Re-emerging Infectious Diseases{\textquoteright} from the Ministry of Health and Welfare, the Government of Japan.",

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doi = "10.1016/S0264-410X(00)00439-4",

language = "English",

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AU - Ohara, Naoya

AU - Matsuoka, Masanori

AU - Nomaguchi, Hiroko

AU - Naito, Mariko

AU - Yamada, Takeshi

N1 - Funding Information: This work was partly supported by grants from the U.S.–Japan Cooperative Medical Science Program and by a ‘Research Grant on Emerging and Re-emerging Infectious Diseases’ from the Ministry of Health and Welfare, the Government of Japan.

PY - 2001/2/28

Y1 - 2001/2/28

N2 - The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

AB - The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.

KW - Ag85

KW - BCG

KW - Leprosy

KW - Recombinant

KW - Vaccine

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DO - 10.1016/S0264-410X(00)00439-4

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AN - SCOPUS:0035961592

SN - 0264-410X

VL - 19

SP - 1906

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JO - Vaccine

JF - Vaccine

IS - 15-16

ER -

Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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