Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses

Bunichi Ezaki; Richard C. Gardner; Yuka Ezaki; Hideki Kondo; Hideaki Matsumoto

doi:10.1016/S0378-1097(97)00554-5

Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses

Bunichi Ezaki, Richard C. Gardner, Yuka Ezaki, Hideki Kondo, Hideaki Matsumoto

Institute of Plant Science and Resources

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Abstract

We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did not; both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast.

Original language	English
Pages (from-to)	99-105
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(97)00554-5
Publication status	Published - Feb 1 1998

Keywords

Aluminum metal stress
HSP150 gene
Heat shock stress
Oxidative stress
SED1 gene
Yeast

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/S0378-1097(97)00554-5

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title = "Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses",

abstract = "We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did not; both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast.",

keywords = "Aluminum metal stress, HSP150 gene, Heat shock stress, Oxidative stress, SED1 gene, Yeast",

author = "Bunichi Ezaki and Gardner, {Richard C.} and Yuka Ezaki and Hideki Kondo and Hideaki Matsumoto",

note = "Funding Information: The authors wish to thank Minoru Murata, Marja Makarow, and Hugh R.B. Pelham for providing strains and plasmid DNAs used in this study. We also thank Bunichirou Ono for his fruitful advice. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 05278106 and No. 05660074), a Grant-in-Aid for Co-operative Research (No. 05340151) from the Ministry of Education, Science, Culture and Sports of Japan, the Ohara Foundation for Agricultural Science and grant 96-AGR-03-5253 from the New Zealand Foundation for Research Science and Technology.",

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doi = "10.1016/S0378-1097(97)00554-5",

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AU - Ezaki, Bunichi

AU - Gardner, Richard C.

AU - Ezaki, Yuka

AU - Kondo, Hideki

AU - Matsumoto, Hideaki

N1 - Funding Information: The authors wish to thank Minoru Murata, Marja Makarow, and Hugh R.B. Pelham for providing strains and plasmid DNAs used in this study. We also thank Bunichirou Ono for his fruitful advice. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (No. 05278106 and No. 05660074), a Grant-in-Aid for Co-operative Research (No. 05340151) from the Ministry of Education, Science, Culture and Sports of Japan, the Ohara Foundation for Agricultural Science and grant 96-AGR-03-5253 from the New Zealand Foundation for Research Science and Technology.

PY - 1998/2/1

Y1 - 1998/2/1

N2 - We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did not; both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast.

AB - We isolated two yeast cDNA clones whose transcripts are induced by aluminum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the other corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the sensitivity of gene-disrupted mutants to Al stress and to oxidative stresses. The Al tests indicated that the HSP150 protein served a basal protective role in Al stress, but SED1 did not; both of the genes had protective roles for oxidative stresses. The results for the HSP150 gene suggest that there is an overlap between Al ion stress, oxidative stress and heat shock stress in yeast.

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KW - HSP150 gene

KW - Heat shock stress

KW - Oxidative stress

KW - SED1 gene

KW - Yeast

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Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses

Abstract

Keywords

ASJC Scopus subject areas

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