TY - JOUR
T1 - Protein Extraction by New Reversed Micelles with Di(Tridecyl) Phosphoric Acid
AU - Goto, Masahiro
AU - Kuroki, Motohiro
AU - Ono, Tsutomu
AU - Nakashio, Fumiy uki
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (No. 0545303) from the Ministry of Education, Science and Culture of Japan. The authors are grateful to Mitsui Cyanamid Co., Ltd., and American Cyanamid Co. for the supply of surfactants AOT and ATR.
PY - 1995
Y1 - 1995
N2 - Di(tridecyl) phosphoric acid (DTDPA) was synthesized as a new surfactant for protein extraction by reversed micelles. DTDPA can form stable reversed micelles in the concentration range above 1 mM. The size of the DTDPA reversed micelles was smaller than that of conventional AOT (sodium-di-2-ethylhexyl sulfosucci-nate) reversed micelles under the same conditions. The formation of reversed micelles using DTDPA can be controlled by adjusting the aqueous pH. The extraction behavior of some proteins from an aqueous phase into the reversed micellar phase of DTDPA has been investigated by varying the experimental conditions (pH, ionic strength, surfactant concentration). It was found that reversed micelles with DTDPA can extract many proteins. In particular, hemoglobin, which is difficult to extract with an AOT reversed micelle, can be extracted in the DTDPA reversed micelles. In the new reversed micellar system, phase separation was very fast even at a high concentration of DTDPA.
AB - Di(tridecyl) phosphoric acid (DTDPA) was synthesized as a new surfactant for protein extraction by reversed micelles. DTDPA can form stable reversed micelles in the concentration range above 1 mM. The size of the DTDPA reversed micelles was smaller than that of conventional AOT (sodium-di-2-ethylhexyl sulfosucci-nate) reversed micelles under the same conditions. The formation of reversed micelles using DTDPA can be controlled by adjusting the aqueous pH. The extraction behavior of some proteins from an aqueous phase into the reversed micellar phase of DTDPA has been investigated by varying the experimental conditions (pH, ionic strength, surfactant concentration). It was found that reversed micelles with DTDPA can extract many proteins. In particular, hemoglobin, which is difficult to extract with an AOT reversed micelle, can be extracted in the DTDPA reversed micelles. In the new reversed micellar system, phase separation was very fast even at a high concentration of DTDPA.
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U2 - 10.1080/01496399508012215
DO - 10.1080/01496399508012215
M3 - Article
AN - SCOPUS:0029219333
SN - 0149-6395
VL - 30
SP - 89
EP - 99
JO - Separation Science and Technology
JF - Separation Science and Technology
IS - 1
ER -