Proteome analysis of new antimalarial endoperoxide against Plasmodium falciparum

Nagwa S.M. Aly; Akiko Hiramoto; Hitomi Sanai; Osamu Hiraoka; Kazuyuki Hiramoto; Hiroyuki Kataoka; Jin Ming Wu; Araki Masuyama; Masatomo Nojima; Satoru Kawai; Hye Sook Kim; Yusuke Wataya

doi:10.1007/s00436-007-0460-8

Proteome analysis of new antimalarial endoperoxide against Plasmodium falciparum

Nagwa S.M. Aly, Akiko Hiramoto, Hitomi Sanai, Osamu Hiraoka, Kazuyuki Hiramoto, Hiroyuki Kataoka, Jin Ming Wu, Araki Masuyama, Masatomo Nojima, Satoru Kawai, Hye Sook Kim, Yusuke Wataya

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

N-89, a new antimalarial endoperoxide, was selected as a promising antimalarial compound showing high activity and selectivity. To study the mechanism of N-89 action, N-89 resistant strain (NRC10) was obtained by intermittent drug pressure. NRC10 had a tenfold increase in the EC₅₀ value of N-89. No cross-resistance was obtained with other antimalarial compounds. Comparative proteome analysis of N-89 sensitive and NRC10 strains revealed over-expression of 12 spots and down-regulation of 14 spots in NRC10. Fifteen proteins were identified of Plasmodium falciparum origin. The identified proteins representing several functions, mainly related to the glycolytic pathway, and metabolism of protein and lipid. Our results suggest that identified proteins may be candidates of antimalarial endoperoxide targets.

Original language	English
Pages (from-to)	1119-1124
Number of pages	6
Journal	Parasitology Research
Volume	100
Issue number	5
DOIs	https://doi.org/10.1007/s00436-007-0460-8
Publication status	Published - Apr 2007

ASJC Scopus subject areas

Parasitology
veterinary(all)
Insect Science
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00436-007-0460-8

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title = "Proteome analysis of new antimalarial endoperoxide against Plasmodium falciparum",

abstract = "N-89, a new antimalarial endoperoxide, was selected as a promising antimalarial compound showing high activity and selectivity. To study the mechanism of N-89 action, N-89 resistant strain (NRC10) was obtained by intermittent drug pressure. NRC10 had a tenfold increase in the EC50 value of N-89. No cross-resistance was obtained with other antimalarial compounds. Comparative proteome analysis of N-89 sensitive and NRC10 strains revealed over-expression of 12 spots and down-regulation of 14 spots in NRC10. Fifteen proteins were identified of Plasmodium falciparum origin. The identified proteins representing several functions, mainly related to the glycolytic pathway, and metabolism of protein and lipid. Our results suggest that identified proteins may be candidates of antimalarial endoperoxide targets.",

author = "Aly, {Nagwa S.M.} and Akiko Hiramoto and Hitomi Sanai and Osamu Hiraoka and Kazuyuki Hiramoto and Hiroyuki Kataoka and Wu, {Jin Ming} and Araki Masuyama and Masatomo Nojima and Satoru Kawai and Kim, {Hye Sook} and Yusuke Wataya",

note = "Funding Information: Acknowledgment This study was supported by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO) (Project No. 04-09, Yusuke Wataya).",

year = "2007",

month = apr,

doi = "10.1007/s00436-007-0460-8",

language = "English",

volume = "100",

pages = "1119--1124",

journal = "Parasitology Research",

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TY - JOUR

T1 - Proteome analysis of new antimalarial endoperoxide against Plasmodium falciparum

AU - Aly, Nagwa S.M.

AU - Hiramoto, Akiko

AU - Sanai, Hitomi

AU - Hiraoka, Osamu

AU - Hiramoto, Kazuyuki

AU - Kataoka, Hiroyuki

AU - Wu, Jin Ming

AU - Masuyama, Araki

AU - Nojima, Masatomo

AU - Kawai, Satoru

AU - Kim, Hye Sook

AU - Wataya, Yusuke

N1 - Funding Information: Acknowledgment This study was supported by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO) (Project No. 04-09, Yusuke Wataya).

PY - 2007/4

Y1 - 2007/4

N2 - N-89, a new antimalarial endoperoxide, was selected as a promising antimalarial compound showing high activity and selectivity. To study the mechanism of N-89 action, N-89 resistant strain (NRC10) was obtained by intermittent drug pressure. NRC10 had a tenfold increase in the EC50 value of N-89. No cross-resistance was obtained with other antimalarial compounds. Comparative proteome analysis of N-89 sensitive and NRC10 strains revealed over-expression of 12 spots and down-regulation of 14 spots in NRC10. Fifteen proteins were identified of Plasmodium falciparum origin. The identified proteins representing several functions, mainly related to the glycolytic pathway, and metabolism of protein and lipid. Our results suggest that identified proteins may be candidates of antimalarial endoperoxide targets.

AB - N-89, a new antimalarial endoperoxide, was selected as a promising antimalarial compound showing high activity and selectivity. To study the mechanism of N-89 action, N-89 resistant strain (NRC10) was obtained by intermittent drug pressure. NRC10 had a tenfold increase in the EC50 value of N-89. No cross-resistance was obtained with other antimalarial compounds. Comparative proteome analysis of N-89 sensitive and NRC10 strains revealed over-expression of 12 spots and down-regulation of 14 spots in NRC10. Fifteen proteins were identified of Plasmodium falciparum origin. The identified proteins representing several functions, mainly related to the glycolytic pathway, and metabolism of protein and lipid. Our results suggest that identified proteins may be candidates of antimalarial endoperoxide targets.

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DO - 10.1007/s00436-007-0460-8

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AN - SCOPUS:33847624986

SN - 0932-0113

VL - 100

SP - 1119

EP - 1124

JO - Parasitology Research

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IS - 5

ER -

Proteome analysis of new antimalarial endoperoxide against Plasmodium falciparum

Abstract

ASJC Scopus subject areas

UN SDGs

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