TY - CHAP
T1 - Proteomic Profiling of the Extracellular Vesicle Chaperone in Cancer
AU - Ono, Kisho
AU - Eguchi, Takanori
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Molecular chaperones are widely distributed intracellular proteins that play essential roles in maintaining proteome function by assisting in the folding of client proteins. Molecular chaperones, such as heat shock proteins (HSPs), are found intracellularly and extracellularly. Extracellular vesicles (EVs), such as exosomes, contain HSPs and horizontally transfer the functional chaperones into various recipient cells. Besides, mass spectrometry has enabled a comprehensive analysis of exosomal and EV proteins, which is useful in basic biomedical research to clinical biomarker search. We have performed deep proteome analysis of EVs, including exosomes, from metastatic tongue and prostate cancers and detected >700 protein types, including cytoplasmic, ER, mitochondrial, small, and large HSPs. Here, we provide protocols for isolating exosomes/EVs and deep proteome analysis to detect the EV chaperone.
AB - Molecular chaperones are widely distributed intracellular proteins that play essential roles in maintaining proteome function by assisting in the folding of client proteins. Molecular chaperones, such as heat shock proteins (HSPs), are found intracellularly and extracellularly. Extracellular vesicles (EVs), such as exosomes, contain HSPs and horizontally transfer the functional chaperones into various recipient cells. Besides, mass spectrometry has enabled a comprehensive analysis of exosomal and EV proteins, which is useful in basic biomedical research to clinical biomarker search. We have performed deep proteome analysis of EVs, including exosomes, from metastatic tongue and prostate cancers and detected >700 protein types, including cytoplasmic, ER, mitochondrial, small, and large HSPs. Here, we provide protocols for isolating exosomes/EVs and deep proteome analysis to detect the EV chaperone.
KW - Chaperone proteins
KW - Exosome isolation methods
KW - Extracellular vesicles
KW - Heat shock protein
KW - Mass spectrometry
KW - Proteome analysis
UR - http://www.scopus.com/inward/record.url?scp=85166558886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85166558886&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3342-7_18
DO - 10.1007/978-1-0716-3342-7_18
M3 - Chapter
C2 - 37540439
AN - SCOPUS:85166558886
T3 - Methods in Molecular Biology
SP - 233
EP - 249
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
