Protocol for Genome Editing to Produce Multiple Mutants in Wheat

Fumitaka Abe, Yuji Ishida, Hiroshi Hisano, Masaki Endo, Toshihiko Komari, Seiichi Toki, Kazuhiro Sato

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Here, we describe a protocol for producing multiple recessive mutants via genome editing in hexaploid wheat (Triticum aestivum) cv. Fielder. Using Agrobacterium-delivered CRISPR/Cas9 and three sub-genome-specific primer sets, all possible combinations of single, double, and triple transgene-free mutants can be generated. The technique for acceleration of generation advancement with embryo culture reduces time for mutant production. The mutants produced by this protocol can be used for the analysis of gene function and crop improvement. For complete details on the use and execution of this protocol, please refer to Abe et al. (2019).

Original languageEnglish
Article number100053
JournalSTAR Protocols
Issue number2
Publication statusPublished - Sept 18 2020

ASJC Scopus subject areas

  • General Biochemistry,Genetics and Molecular Biology
  • General Neuroscience
  • General Immunology and Microbiology


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