TY - JOUR
T1 - PBAD-based shuttle vectors for functional analysis of toxic and highly regulated genes in Pseudomonas and Burkholderia spp. and other bacteria
AU - Qiu, Dongru
AU - Damron, F. Heath
AU - Mima, Takehiko
AU - Schweizer, Herbert P.
AU - Yu, Hongwei D.
PY - 2008/12
Y1 - 2008/12
N2 - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.
AB - We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.
UR - http://www.scopus.com/inward/record.url?scp=57149099185&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57149099185&partnerID=8YFLogxK
U2 - 10.1128/AEM.01369-08
DO - 10.1128/AEM.01369-08
M3 - Article
C2 - 18849445
AN - SCOPUS:57149099185
SN - 0099-2240
VL - 74
SP - 7422
EP - 7426
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 23
ER -