PtdIns4 KIIα generates endosomal PtdIns(4)P and is required for receptor sorting at early endosomes

Yuji Henmi, Yoshiaki Morikawa, Natsuko Oe, Narumi Ikeda, Akikazu Fujita, Kohji Takei, Shane Minogue, Kenji Tanabe

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


Phosphatidylinositol 4-kinase IIα (PtdIns4KIIα) localizes to the trans-Golgi network and endosomal compartments and has been implicated in the regulation of endosomal traffic, but the roles of both its enzymatic activity and the site of its action have not been elucidated. This study shows that PtdIns4KIIα is required for production of endosomal phosphatidylinositol 4-phosphate (PtdIns(4)P) on early endosomes and for the sorting of transferrin and epidermal growth factor receptor into recycling and degradative pathways. Depletion of PtdIns4KIIα with small interfering RNA significantly reduced the amount of vesicular PtdIns(4)P on early endosomes but not on Golgi membranes. Cells depleted of PtdIns4KIIα had an impaired ability to sort molecules destined for recycling from early endosomes. We further identify the Eps15 homology domaincontaining protein 3 (EHD3) as a possible endosomal effector of PtdIns4KIIα. Tubular endosomes containing EHD3 were shortened and became more vesicular in PtdIns4KIIα-depleted cells. Endosomal PtdIns(4,5)P2 was also significantly reduced in PtdIns4KIIα-depleted cells. These results show that PtdIns4KIIα regulates receptor sorting at early endosomes through a PtdIns(4)P-dependent pathway and contributes substrate for the synthesis of endosomal PtdIns(4,5)P2.

Original languageEnglish
Pages (from-to)990-1001
Number of pages12
JournalMolecular Biology of the Cell
Issue number6
Publication statusPublished - Mar 15 2016

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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