PuraMatrix™ facilitates bone regeneration in bone defects of calvaria in mice

Haruo Misawa, Naoya Kobayashi, Alejandro Soto-Gutierrez, Yong Chen, Aki Yoshida, Jorge David Rivas-Carrillo, Nalu Navarro-Alvarez, Kimiaki Tanaka, Atsushi Miki, Jiro Takei, Tadayoshi Ueda, Masato Tanaka, Hirosuke Endo, Noriaki Tanaka, Toshifumi Ozaki

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)


Artificial bones have often used for bone regeneration due to their strength, but they cannot provide an adequate environment for cell penetration and settlement. We therefore attempted to explore various materials that may allow the cells to penetrate and engraft in bone defects. PuraMatrix™ is a self-assembling peptide scaffold that produces a nanoscale environment allowing both cellular penetration and engraftment. The objective of this study was to investigate the effect of PuraMatrix™ on bone regeneration in a mouse bone defect model of the calvaria. Matrigel™ was used as a control. The expression of bone-related genes (alkaline phosphatase, Runx2, and Osterix) in the PuraMatrix™-injected bone defects was stronger than that in the Matrigel™-injected defects. Soft X-ray radiographs revealed that bony bridges were clearly observed in the defects treated with PuraMatrix™, but not in the Matrigel™-treated defects. Notably, PuraMatrix™ treatment induced mature bone tissue while showing cortical bone medullary cavities. The area of newly formed bones at the site of the bone defects was 1.38-fold larger for PuraMatrix™ than Matrigel™. The strength of the regenerated bone was 1.72-fold higher for PuraMatrix™ (146.0 g) than for Matrigel™ (84.7 g). The present study demonstrated that PuraMatrix™ injection favorably induced functional bone regeneration.

Original languageEnglish
Pages (from-to)903-910
Number of pages8
JournalCell Transplantation
Issue number10
Publication statusPublished - 2006


  • Bone regeneration
  • Calvaria
  • Matrigel™
  • PuraMatrix™

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation


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