TY - JOUR
T1 - Purification and crystallization of oxygen-evolving photosystem II core complex from thermophilic cyanobacteria.
AU - Shen, Jian Ren
AU - Kawakami, Keisuke
AU - Koike, Hiroyuki
PY - 2011
Y1 - 2011
N2 - This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
AB - This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus.
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U2 - 10.1007/978-1-60761-925-3_5
DO - 10.1007/978-1-60761-925-3_5
M3 - Article
C2 - 20960120
AN - SCOPUS:79952115457
SN - 1064-3745
VL - 684
SP - 41
EP - 51
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -