Quantitation of glutathione S transferase-π in the urine of preterm neonates

Hirokazu Tsukahara, Michiyo Toyo-Oka, Yuko Kanaya, Kazumasa Ogura, Masao Kawatani, Atsuko Hata, Masahiro Hiraoka, Mitsufumi Mayumi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Background: Glutathione S transferases (GSTs) are widely distributed enzymes found in highly varying amounts in tissues of the human body. The enzyme GST-π in urine has been used as a marker of renal distal tubular cell damage. The present study was intended to evaluate urinary excretion of GST-π and its relationship to other renal markers and to the status of oxidative stress in preterm neonates. Methods: Levels of urinary GST-π, N-acetyl-β-glucosaminidase (a marker of proximal tubular damage), albumin (a marker of glomerular damage) and 8-hydroxy-2′-deoxyguanosine (a marker of oxidative stress) and serum creatinine were measured in preterm neonates at 1 and 4 weeks of age. Results: The results showed that urinary excretion of GST-π is increased in preterm neonates compared with reported values for healthy adults. No significant relationship was detected between urinary GST-π and other markers for renal function. Urinary GST-π showed significantly positive correlation with urinary 8-hydroxy-2′- deoxyguanosine at 1 and 4 weeks. Sick neonates treated with supplemental oxygen and mechanical ventilation showed significantly higher levels of GST-π as well as 8-hydroxy-2′-deoxyguanosine than clinically stable neonates did at 4 weeks. Conclusions: These results indicate the potential effect of systemic oxidative stress on urinary excretion of GST-π. Further studies are necessary to explore the effect of oxidative conditions on expression of GST-π in distal tubules in the human kidney.

Original languageEnglish
Pages (from-to)528-531
Number of pages4
JournalPediatrics International
Issue number5
Publication statusPublished - Oct 2005
Externally publishedYes


  • 8-hydroxy-2′-deoxyguanosine
  • Glutathione S transferase-π
  • Oxidative stress
  • Preterm neonate

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health


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