RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato

Taku Ohmori; Minora Murata; Fusao Motoyoshi

doi:10.1266/jjg.70.179

RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato

Taku Ohmori, Minora Murata, Fusao Motoyoshi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC₁ plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.

Original language	English
Pages (from-to)	179-184
Number of pages	6
Journal	Japanese Journal of Genetics
Volume	70
Issue number	2
DOIs	https://doi.org/10.1266/jjg.70.179
Publication status	Published - 1995

ASJC Scopus subject areas

Genetics

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title = "RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato",

abstract = "Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC1 plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.",

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AU - Ohmori, Taku

AU - Murata, Minora

AU - Motoyoshi, Fusao

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Y1 - 1995

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AB - Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC1 plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.

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RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato

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