TY - JOUR
T1 - Rapid Isolation of Endogenous Inhibitor of Factor B
AU - Moriyama, Takeshi
AU - Kawamoto, Yasuko
AU - Kobayashi, Tatsuhiko
AU - Inoue, Takashi
AU - Okamoto, Keinosuke
AU - Mivama, Akio
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1984
Y1 - 1984
N2 - An inhibitor of mouse factor B was purified from lysate of L cells by a human factor B-affinity column. The purified inhibitor was found to be homogeneous with a molecular weight of about 25,000 and identical to the 25K protein isolated from L cell-liysate by the coprecipitation method with anti mouse factor B. Hemolytic titration of the activity and PAGE-analysis of the inhibitor-factor B complex showed that 1 mol of inhibitor reacted with 1 mol of mouse factor B and inactivated its hemolytic activity.
AB - An inhibitor of mouse factor B was purified from lysate of L cells by a human factor B-affinity column. The purified inhibitor was found to be homogeneous with a molecular weight of about 25,000 and identical to the 25K protein isolated from L cell-liysate by the coprecipitation method with anti mouse factor B. Hemolytic titration of the activity and PAGE-analysis of the inhibitor-factor B complex showed that 1 mol of inhibitor reacted with 1 mol of mouse factor B and inactivated its hemolytic activity.
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U2 - 10.1111/j.1348-0421.1984.tb00686.x
DO - 10.1111/j.1348-0421.1984.tb00686.x
M3 - Article
C2 - 6564347
AN - SCOPUS:0021270060
SN - 0385-5600
VL - 28
SP - 349
EP - 357
JO - MICROBIOLOGY and IMMUNOLOGY
JF - MICROBIOLOGY and IMMUNOLOGY
IS - 3
ER -