Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

Tadashi Ema; Toshiyuki Fujii; Misa Ozaki; Toshinobu Korenaga; Takashi Sakai

doi:10.1039/b508244g

Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

Tadashi Ema, Toshiyuki Fujii, Misa Ozaki, Toshinobu Korenaga, Takashi Sakai

School of Engineering

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.

Original language	English
Pages (from-to)	4650-4651
Number of pages	2
Journal	Chemical Communications
Issue number	37
DOIs	https://doi.org/10.1039/b508244g
Publication status	Published - Oct 7 2005

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/b508244g

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title = "Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism",

abstract = "The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.",

author = "Tadashi Ema and Toshiyuki Fujii and Misa Ozaki and Toshinobu Korenaga and Takashi Sakai",

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AU - Ema, Tadashi

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AU - Ozaki, Misa

AU - Korenaga, Toshinobu

AU - Sakai, Takashi

PY - 2005/10/7

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

Abstract

ASJC Scopus subject areas

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