Rational design of peptide-functionalized surface plasmon resonance sensor for specific detection of TNT explosive

Jin Wang, Masaki Muto, Rui Yatabe, Takeshi Onodera, Masayoshi Tanaka, Mina Okochi, Kiyoshi Toko

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


In this study, a rationally-designed 2,4,6-trinitrotoluene (TNT) binding peptide derived from an amino acid sequence of the complementarity-determining region (CDR) of an anti-TNT monoclonal antibody was used for TNT detection based on a maleimide-functionalized surface plasmon resonance (SPR) sensor. By antigen-docking simulation and screening, the TNT binding candidate peptides were obtained as TNTHCDR1 derived from the heavy chain of CDR1, TNTHCDR2 derived from CDR2, and TNTHCDR3 from CDR3 of an anti-TNT antibody. The binding events between candidate peptides and TNT were evaluated using the SPR sensor by direct determination based on the 3-aminopropyltriethoxysilane (APTES) surface. The TNT binding peptide was directly immobilized on the maleimide-functionalized sensor chip surface from N-γ-maleimidobutyryl-oxysuccinimide ester (GMBS). The results demonstrated that peptide TNTHCDR3 was identified and selected as a TNT binding peptide among the other two candidate peptides. Five kinds of TNT analogues were also investigated to testify the selectivity of TNT binding peptide TNTHCDR3. Furthermore, the results indicated that the APTES-GMBS-based SPR sensor chip procedure featured a great potential application for the direct detection of TNT.

Original languageEnglish
Article number2249
JournalSensors (Switzerland)
Issue number10
Publication statusPublished - Oct 2017
Externally publishedYes


  • Amino acid sequence
  • N-γ-maleimidobutyryl-oxysuccinimide ester
  • Rationally designed
  • Surface plasmon resonance
  • TNT binding peptides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering


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