Reaction between Graphene Oxide and Intracellular Glutathione Affects Cell Viability and Proliferation

Baojin Ma, Shi Guo, Yuta Nishina, Alberto Bianco

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Graphene oxide (GO) is currently developed for biomedical applications as a promising nanoplatform for drug delivery, phototherapy, and biosensing. As a consequence, its safety and cytotoxicity issues have attracted extensive attention. It has been demonstrated that GO causes an increase of intracellular oxidative stress, likely leading to its cytotoxicity and inhibition of cell proliferation. Being one of the main reductive intracellular substances, glutathione (GSH) is vital in the regulation of the oxidative stress level to maintain normal cellular functions. In this study, we found that GSH could be oxidized to GSSG by GO, leading to the formation of reduced GO (rGO). GSH depletion affects the intracellular reductive/oxidative balance, provoking the increase of the reactive oxygen species level, sequentially inhibiting cell viability and proliferation. Therefore, the reaction between GO and GSH provides a new perspective to explain the origin of GO cytotoxicity.

Original languageEnglish
Pages (from-to)3528-3535
Number of pages8
JournalACS Applied Materials and Interfaces
Issue number3
Publication statusPublished - Jan 27 2021


  • cancer cells
  • carbon nanomaterial
  • oxidation
  • reactive oxygen species
  • reduction

ASJC Scopus subject areas

  • Materials Science(all)


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