TY - JOUR
T1 - Regional differences in the expression of DNA topoisomerase IIβ in the pyramidal neurons of the rat hippocampus
AU - Maeda, Yukihide
AU - Tsutsui, Kimiko
AU - Tsutsui, Ken
AU - Tokunaga, Akira
N1 - Funding Information:
We thank Professor A. Kikuchi (Laboratory of Med. Mycology, Research Institute for Disease Mechanism and Control, Nagoya Univ., School of Med.) for providing the specific monoclonal antibody to topo IIβ (3B6). We are also grateful to Dr Sepehr Eskandari for his critical reading of the manuscript. We thank Ms Reiko Takeuchi for her technical assistance. This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2000/4
Y1 - 2000/4
N2 - A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - A detailed analysis of the differential expression of a nuclear enzyme, DNA topoisomerase (topo) IIβ, was performed in the rat hippocampal pyramidal layer. Three-dimensional (3-D) reconstruction from serial sections immunostained with anti-topo IIβ antibody showed that the immunoreactivity was apparently weak in the entire CA3 region. Almost all CA1 pyramidal cells showed similar immunoreactivity to that seen in the dentate granular cells, the subicular neurons, and the cerebral neocortical neurons. In addition, immunoblotting analysis in the adult dorsal hippocampus showed that the expression level of topo IIβ in the CA3 was significantly lower than that in the CA1 region. The dissociation in the expression level between CA1 and CA3 occurred in postnatal days 4 (P4) through P6. The present finding suggests that the enzyme is possibly involved in activities of the hippocampal pyramidal neurons. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - 3-D reconstruction
KW - DNA topoisomerase IIβ
KW - Hippocampal pyramidal cells
KW - Immunohistochemistry
KW - Postnatal development
KW - Western blotting
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U2 - 10.1016/S0168-0102(00)00098-5
DO - 10.1016/S0168-0102(00)00098-5
M3 - Article
C2 - 10771107
AN - SCOPUS:0034069863
SN - 0168-0102
VL - 36
SP - 291
EP - 296
JO - Neuroscience Research
JF - Neuroscience Research
IS - 4
ER -