Abstract
We searched for miRNAs that were down-regulated in chondrocytic cells and predicted to target CCN2/connective tissue growth factor (CCN2/CTGF) that promotes endochondral ossification. Among them, expression of miR-18a was most strongly repressed in chondrocytic cells. Reporter gene analysis confirmed the functionality of an miR-18a target in the 3′-untranslated region of Ccn2 mRNA, which was predicted in silico. Indeed, introduction of miR-18a efficiently repressed the CCN2 production from chondrocytic cells. Finally, transfected miR-18a significantly repressed the mature chondrocytic phenotype. Our present study revealed a regulatory role for miR-18a in chondrocytic differentiation through CCN2.
Original language | English |
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Pages (from-to) | 1006-1010 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 583 |
Issue number | 6 |
DOIs | |
Publication status | Published - Mar 18 2009 |
Keywords
- CCN family
- Chondrocyte
- Connective tissue growth factor
- Micro RNA
- miR-18a
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology