Regulation of sulphate assimilation in Saccharomyces cerevisiae

Bun Ichiro Ono, Kazuyasu Kijima, Nobuya Ishii, Takahiro Kawato, Akio Matsuda, Andrzej Paszewski, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


We examined how the activity of O-acetylserine and O-acetylhomoserine sulphydrylase (OAS/OAH) SHLase of Saccharomyces cerevisiae is affected by sulphur source added to the growth medium and genetic background of the strain. In a wild-type strain, the activity was repressed if methionine, cysteine or glutathione was added to the growth medium. However, in a strain deficient of cystathionine γ-lyase, cysteine and glutathione were repressive, but methionine was not. In strains deficient of serine O-acetyltransferase (SATase), OAS/OAH SHLase activity was low regardless of sulphur source and was further lowered by cysteine and glutathione, but not by methionine. From these observations, we concluded that S-adenosylmethionine should be excluded from being the effector for regulation of OAS/OAH SHLase. Instead, we suspected that S. cerevisiae would have the same regulatory system as Escherichia coli for sulphate assimilation; i.e. cysteine inhibits SATase to lower the cellular concentration of OAS which is required for induction of the sulphate assimilation enzymes including OAS/OAH SHLase. Subsequently, we obtained data supporting this speculation.

Original languageEnglish
Pages (from-to)1153-1162
Number of pages10
Issue number11
Publication statusPublished - Sept 15 1996
Externally publishedYes


  • Cysteine regulon
  • O-acetylserine
  • Saccharomyces cerevisiae
  • Serine O-acetyltransferase
  • Sulphate assimilation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics


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