Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells

Hiromu Kawasaki, Hayato Hino, Fusako Takayama, Yoshihisa Kitamura, Toshiaki Sendou, Shingo Takatori

Research output: Contribution to journalArticlepeer-review


We have reported that nicotine has a neurotrophic action on peripheral adrenergic nerves in vivo, which is mediated by α7 nicotinic acetylcholine receptors (nAChRs). To clarify the possible mechanisms, the present study further investigated the effect of nicotine on neurite outgrowth in tyrosine hydroxylase (TH)-positive superior cervical ganglia (SCG) cells isolated from neonatal rats in vitro. Nicotine at low concentrations (0.01–0.3 mM) increased the number of neurite outgrowths in TH-immunopositive SCG cells, while high concentrations of nicotine (1–10 mM) gradually reduced it, and only 10 mM nicotine was markedly inhibited compared to the control. A 100 μM of nicotine-induced increase in neurite numbers depended on the exposure time and was inhibited by treatment with the nAChR antagonist hexamethonium (Hex) and α7 nAChR antagonist α-bungarotoxin (α-Bgtx). The nicotine (10 mM)-induced a significant decrease in neurite outgrowth in SCG, which was perfectly canceled by Hex to the control level but not by α-Bgtx. These results suggest that nicotine has a regulatory neurotrophic action mediated by both α7 nAChR and other subtypes in TH-positive SCG cells of rats.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalJournal of Pharmacological Sciences
Issue number1
Publication statusPublished - Jan 2022


  • Neurite outgrowth
  • Nicotine
  • Nicotinic acetylcholine receptors
  • Rat superior cervical ganglia cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology


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