Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint

Yuriko Hirota; Manabu Habu; Kazuhiro Tominaga; Miho Sukedai; Akihiro Matsukawa; Tatsuji Nishihara; Jinichi Fukuda

doi:10.1111/j.1600-0714.2006.00367.x

Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint

Yuriko Hirota, Manabu Habu, Kazuhiro Tominaga, Miho Sukedai, Akihiro Matsukawa, Tatsuji Nishihara, Jinichi Fukuda

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

BACKGROUND: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthritis. METHOD: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF-α and TUNEL was performed. RESULTS: In condylar cartilage, TNF-α-positive cells and TUNEL-positive cells were localized together. TNF-α-positive chondrocytes seemed to precede TUNEL-positive cells. CONCLUSIONS: The results of the present study suggest that TNF-α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.

Original language	English
Pages (from-to)	91-98
Number of pages	8
Journal	Journal of Oral Pathology and Medicine
Volume	35
Issue number	2
DOIs	https://doi.org/10.1111/j.1600-0714.2006.00367.x
Publication status	Published - Feb 1 2006
Externally published	Yes

Keywords

Antigen-induced arthritis
Apoptosis
Rabbit
Temporomandibular joint
Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling
Tumor necrosis factor-α

ASJC Scopus subject areas

Pathology and Forensic Medicine
Oral Surgery
Otorhinolaryngology
Cancer Research
Periodontics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1600-0714.2006.00367.x

Cite this

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title = "Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint",

abstract = "BACKGROUND: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthritis. METHOD: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF-α and TUNEL was performed. RESULTS: In condylar cartilage, TNF-α-positive cells and TUNEL-positive cells were localized together. TNF-α-positive chondrocytes seemed to precede TUNEL-positive cells. CONCLUSIONS: The results of the present study suggest that TNF-α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.",

keywords = "Antigen-induced arthritis, Apoptosis, Rabbit, Temporomandibular joint, Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling, Tumor necrosis factor-α",

author = "Yuriko Hirota and Manabu Habu and Kazuhiro Tominaga and Miho Sukedai and Akihiro Matsukawa and Tatsuji Nishihara and Jinichi Fukuda",

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doi = "10.1111/j.1600-0714.2006.00367.x",

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T1 - Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint

AU - Hirota, Yuriko

AU - Habu, Manabu

AU - Tominaga, Kazuhiro

AU - Sukedai, Miho

AU - Matsukawa, Akihiro

AU - Nishihara, Tatsuji

AU - Fukuda, Jinichi

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N2 - BACKGROUND: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthritis. METHOD: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF-α and TUNEL was performed. RESULTS: In condylar cartilage, TNF-α-positive cells and TUNEL-positive cells were localized together. TNF-α-positive chondrocytes seemed to precede TUNEL-positive cells. CONCLUSIONS: The results of the present study suggest that TNF-α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.

AB - BACKGROUND: Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling (TUNEL) staining is a widely accepted method for the detection of DNA fragmentation in nuclei of apoptotic cells. Tumor necrosis factor (TNF)-α is closely associated with changes in condylar cartilage and modulates apoptosis in various tissues including cartilage. The aim of this study was to investigate the relationship between apoptotic chondrocytes and TNF-α in a rabbit model of arthritis. METHOD: Unilateral temporomandibular joint (TMJ) arthritis was induced in 20 adult New Zealand White rabbits. From 1 day to 6 weeks after the induction of arthritis, immunohistochemical analysis for TNF-α and TUNEL was performed. RESULTS: In condylar cartilage, TNF-α-positive cells and TUNEL-positive cells were localized together. TNF-α-positive chondrocytes seemed to precede TUNEL-positive cells. CONCLUSIONS: The results of the present study suggest that TNF-α may be involved in apoptosis and/or apoptotic necrosis of chondrocytes as TMJ arthritis progresses from the acute to chronic stage.

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KW - Apoptosis

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KW - Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate biotin nick end-labeling

KW - Tumor necrosis factor-α

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Relationship between TNF-α and TUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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