TY - JOUR
T1 - Reperfusion hastens appearance and extent of distribution of type I collagen in infarct zone
T2 - Immunohistochemical study in rat experimental infarction
AU - Yamasaki, S.
AU - Kusachi, S.
AU - Moritani, H.
AU - Kondo, J.
AU - Hirohata, S.
AU - Tamura, A.
AU - Tsuji, T.
PY - 1995
Y1 - 1995
N2 - Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.
AB - Background: The effects of reperfusion on time-dependent alteration of type I collagen have not been examined. Objectives: We compared the sequential changes in the appearance and distribution of type I collagen in reperfused infarct rat hearts to those in non-reperfused hearts. Methods: Using an experimental rat model of infarction, we performed immunohistochemical staining with a polyclonal antibody to type I collagen by the avidin-biotin-peroxidase method. Reperfusion was established after 2-h coronary ligation that produced complete necrosis of myocytes. Results: In reperfused hearts, type I collagen appeared in the peripheral zone of the infarct at day 2, which was 1 day earlier than in non-reperfused hearts. The extent of distribution of type I collagen in reperfused hearts was comparable to that observed approximately 1 day later in non-reperfused hearts. Conclusion: Reperfusion can accelerate collagen matrix formation compared with that in non-reperfused hearts after acute myocardial infarction.
KW - Avidin-biotin-peroxidase method
KW - Extracellular matrix
KW - Myocardial fibrosis
KW - Myocardial infarct healing
KW - Rat, anesthetized
KW - Reperfusion
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U2 - 10.1016/0008-6363(95)00116-6
DO - 10.1016/0008-6363(95)00116-6
M3 - Article
C2 - 8595624
AN - SCOPUS:0028793558
SN - 0008-6363
VL - 30
SP - 763
EP - 768
JO - Cardiovascular Research
JF - Cardiovascular Research
IS - 5
ER -