Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A

Yoshihito Kitaoku; Gaston Courtade; Dejan M Petrović; Tamo Fukamizo; Vincent G H Eijsink; Finn L Aachmann

doi:10.1007/s12104-018-9839-y

Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A

Yoshihito Kitaoku, Gaston Courtade, Dejan M Petrović, Tamo Fukamizo, Vincent G H Eijsink, Finn L Aachmann

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.

Original language	English
Pages (from-to)	357-361
Number of pages	5
Journal	Biomolecular NMR Assignments
Volume	12
Issue number	2
DOIs	https://doi.org/10.1007/s12104-018-9839-y
Publication status	Published - Oct 2018

Keywords

Apoenzymes/chemistry
Cellulose/metabolism
Mixed Function Oxygenases/chemistry
Nuclear Magnetic Resonance, Biomolecular
Thermoascus/enzymology

Access to Document

10.1007/s12104-018-9839-y

Cite this

@article{ade35ed3cf3c42e0944b472f4505bbe5,

title = "Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A",

abstract = "The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.",

keywords = "Apoenzymes/chemistry, Cellulose/metabolism, Mixed Function Oxygenases/chemistry, Nuclear Magnetic Resonance, Biomolecular, Thermoascus/enzymology",

author = "Yoshihito Kitaoku and Gaston Courtade and Petrovi{\'c}, {Dejan M} and Tamo Fukamizo and Eijsink, {Vincent G H} and Aachmann, {Finn L}",

year = "2018",

month = oct,

doi = "10.1007/s12104-018-9839-y",

language = "English",

volume = "12",

pages = "357--361",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A

AU - Kitaoku, Yoshihito

AU - Courtade, Gaston

AU - Petrović, Dejan M

AU - Fukamizo, Tamo

AU - Eijsink, Vincent G H

AU - Aachmann, Finn L

PY - 2018/10

Y1 - 2018/10

N2 - The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.

AB - The apo-form of the 24.4 kDa AA9 family lytic polysaccharide monooxygenase TaLPMO9A from Thermoascus aurantiacus has been isotopically labeled and recombinantly expressed in Pichia pastoris. In this paper, we report the 1H, 13C, and 15N chemical shift assignments, as well as an analysis of the secondary structure of the protein based on the secondary chemical shifts.

KW - Apoenzymes/chemistry

KW - Cellulose/metabolism

KW - Mixed Function Oxygenases/chemistry

KW - Nuclear Magnetic Resonance, Biomolecular

KW - Thermoascus/enzymology

U2 - 10.1007/s12104-018-9839-y

DO - 10.1007/s12104-018-9839-y

M3 - Article

C2 - 30117034

SN - 1874-2718

VL - 12

SP - 357

EP - 361

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 2

ER -

Resonance assignments for the apo-form of the cellulose-active lytic polysaccharide monooxygenase TaLPMO9A

Abstract

Keywords

Access to Document

Fingerprint

Cite this