Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

Kazuyuki Hirooka; Masaaki Tokuda; Toyohiro Tsumura; Bushra Y. Ahmed; Toshifumi Itano; Hideki Matsui; Ryoji Konishi; Eiichi Hasegawa; Sachiko Okuno; Takako Kitani; Hitoshi Fujisawa; Osamu Hatase

doi:10.1016/S0042-6989(97)00175-2

Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

Kazuyuki Hirooka, Masaaki Tokuda, Toyohiro Tsumura, Bushra Y. Ahmed, Toshifumi Itano, Hideki Matsui, Ryoji Konishi, Eiichi Hasegawa, Sachiko Okuno, Takako Kitani, Hitoshi Fujisawa, Osamu Hatase

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca²⁺/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

Original language	English
Pages (from-to)	2029-2033
Number of pages	5
Journal	Vision Research
Volume	37
Issue number	15
DOIs	https://doi.org/10.1016/S0042-6989(97)00175-2
Publication status	Published - Aug 1997

Keywords

Ca-calmodulin-dependent protein kinase IV
Reticalmin
Retina

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1016/S0042-6989(97)00175-2

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@article{d163b5c005154e1899202a51587e04da,

title = "Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina",

abstract = "Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.",

keywords = "Ca-calmodulin-dependent protein kinase IV, Reticalmin, Retina",

author = "Kazuyuki Hirooka and Masaaki Tokuda and Toyohiro Tsumura and Ahmed, {Bushra Y.} and Toshifumi Itano and Hideki Matsui and Ryoji Konishi and Eiichi Hasegawa and Sachiko Okuno and Takako Kitani and Hitoshi Fujisawa and Osamu Hatase",

note = "Funding Information: Acknowledgements--We are grateful to Dr Najma Janjua and Mr Keith Philibert for their proofreading of this manuscript. We thank the staffs of the Institute for Radioisotopes and the Common Equipment Research Center of the Kagawa Medical University for their technical assistance. This work was partly supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.",

year = "1997",

month = aug,

doi = "10.1016/S0042-6989(97)00175-2",

language = "English",

volume = "37",

pages = "2029--2033",

journal = "Vision Research",

issn = "0042-6989",

publisher = "Elsevier Limited",

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TY - JOUR

T1 - Reticalmin

T2 - A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

AU - Hirooka, Kazuyuki

AU - Tokuda, Masaaki

AU - Tsumura, Toyohiro

AU - Ahmed, Bushra Y.

AU - Itano, Toshifumi

AU - Matsui, Hideki

AU - Konishi, Ryoji

AU - Hasegawa, Eiichi

AU - Okuno, Sachiko

AU - Kitani, Takako

AU - Fujisawa, Hitoshi

AU - Hatase, Osamu

N1 - Funding Information: Acknowledgements--We are grateful to Dr Najma Janjua and Mr Keith Philibert for their proofreading of this manuscript. We thank the staffs of the Institute for Radioisotopes and the Common Equipment Research Center of the Kagawa Medical University for their technical assistance. This work was partly supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.

PY - 1997/8

Y1 - 1997/8

N2 - Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

AB - Western blot analysis of 100,000g supernatant of rat retina using a polyclonal anti-Ca2+/calmodulin-dependent protein kinase IV (CaM-kinase IV) antibody revealed an immunoreactive mass of 35 kDa, termed reticalmin. Lower amount of a isoform of CaM-kinase IV was also expressed in rat retina. Reticalmin did not react with anti-CaM-kinase IV C-terminal peptide antibody which recognized α and β isoforms of CaM-kinase IV and calspermin. Immunohistochemically reticalmin was shown to be localized mainly in the outer segment of photo-receptor cells, and in dendrites of inner plexiform layers and may be in nuclei of ganglion cells and some inner nuclear layer cells.

KW - Ca-calmodulin-dependent protein kinase IV

KW - Reticalmin

KW - Retina

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Reticalmin: A novel calcium-calmodulin-dependent protein kinase IV-like protein in rat retina

Abstract

Keywords

ASJC Scopus subject areas

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