Reversible effects of sodium butyrate on the differentiation of F9 embryonal carcinoma cells

Mitsuko Kosaka, Yukio Nishina, Masashi Takeda, Keishi Matsumoto, Yoshitake Nishimune

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28 Citations (Scopus)


We have studied effects of sodium butyrate on embryonal carcinoma F9 cell differentiation. In the presence of sodium butyrate, F9 cells underwent rapid and drastic morphological changes and expressed marked increases in mRNA levels of various differentiation markers. When sodium butyrate was removed from the cultures, all the examined phenotypes of F9 cell differentiation rapidly reverted to the characteristics of undifferentiated stem cells. However, under the same conditions, when cycloheximide or actinomycin D was added to the cultures, such phenotypic reversion was not observed, but high mRNA levels of the differentiation markers as well as altered cell morphology were retained. These results indicated that the effects of sodium butyrate on induction of teratocarcinoma cell differentiation were reversible and that de novo syntheses of some mRNA(s) and protein(s) were necessary for the reversion of differentiated cells to stem cells.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalExperimental Cell Research
Issue number1
Publication statusPublished - Jan 1991
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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