Robustness analysis of cellular systems using the genetic tug-of-war method

Hisao Moriya, Koji Makanae, Kenji Watanabe, Ayako Chino, Yuki Shimizu-Yoshida

Research output: Contribution to journalReview articlepeer-review

21 Citations (Scopus)


Robustness is one of the principles of design inherent to biological systems. Cellular robustness can be measured as limits of intracellular parameters such as gene expression levels. We have recently developed an experimental approach coined as genetic Tug-Of-War (gTOW), which we used to perform robustness analysis in yeast. Using gTOW, we were able to measure the upper limit of expression of gene targets. In this review, we first elaborate on how the gTOW method compares to current mathematical simulation models prevalently used in the determination of robustness. We then explain the experimental principles underlying gTOW and its associated tools, and we provide concrete examples of robustness analysis using gTOW, i.e. cell cycle and HOG pathway gene expression analysis. Finally, we list a series of Q&As related to the experimental utilization of gTOW and we describe the potential impact of gTOW and its relevance to the understanding of biological systems.

Original languageEnglish
Pages (from-to)2513-2522
Number of pages10
JournalMolecular BioSystems
Issue number10
Publication statusPublished - Oct 2012

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology


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