TY - JOUR
T1 - Role of chicken Pit-1 isoforms in activating growth hormone gene
AU - Murase, Daisuke
AU - Taniuchi, Shusuke
AU - Takeuchi, Sakae
AU - Adachi, Hiromi
AU - Kansaku, Norio
AU - Okazaki, Katsuichiro
AU - Ohkubo, Takeshi
PY - 2011/9/1
Y1 - 2011/9/1
N2 - In the present study, we expressed chicken (ch) Pit-1α (chPit-1α) and chPit-1γ in vitro to compare the roles of chPit-1s in the transcription of the chicken growth hormone (chGH) gene. Both green fluorescence protein (GFP)-fused chPit-1γ and GFP-fused chPit-1α were localized in the nuclei of COS-7 cells. In a luciferase reporter gene assay, both chPit-1α and chPit-1γ transactivated the chGH promoter, and chPit-1α showed a more potent effect than chPit-1γ. On the other hand, an increase of cellular cAMP induced by forskolin promoted transactivation of the chGH gene with chPit-1α and chPit-1γ to similar extents. These results suggest that chPit-1γ may modulate the basal promoter activity of the chGH gene to the same degree as chPit-1α; however, a structural difference observed at the N-terminus transactivation domains in chPit-1α and chPit-1γ could be associated with the efficiency of basal activation of the chGH promoter.
AB - In the present study, we expressed chicken (ch) Pit-1α (chPit-1α) and chPit-1γ in vitro to compare the roles of chPit-1s in the transcription of the chicken growth hormone (chGH) gene. Both green fluorescence protein (GFP)-fused chPit-1γ and GFP-fused chPit-1α were localized in the nuclei of COS-7 cells. In a luciferase reporter gene assay, both chPit-1α and chPit-1γ transactivated the chGH promoter, and chPit-1α showed a more potent effect than chPit-1γ. On the other hand, an increase of cellular cAMP induced by forskolin promoted transactivation of the chGH gene with chPit-1α and chPit-1γ to similar extents. These results suggest that chPit-1γ may modulate the basal promoter activity of the chGH gene to the same degree as chPit-1α; however, a structural difference observed at the N-terminus transactivation domains in chPit-1α and chPit-1γ could be associated with the efficiency of basal activation of the chGH promoter.
KW - Chicken
KW - Growth hormone
KW - Pit-1
KW - Promoter
UR - http://www.scopus.com/inward/record.url?scp=79961127546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961127546&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2011.06.007
DO - 10.1016/j.ygcen.2011.06.007
M3 - Article
C2 - 21703269
AN - SCOPUS:79961127546
SN - 0016-6480
VL - 173
SP - 248
EP - 252
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 2
ER -