TY - JOUR
T1 - Role of neuron-derived ATP in paclitaxel-induced HMGB1 release from macrophages and peripheral neuropathy
AU - Domoto, Risa
AU - Sekiguchi, Fumiko
AU - Kamaguchi, Riki
AU - Iemura, Maiko
AU - Yamanishi, Hiroki
AU - Tsubota, Maho
AU - Wang, Dengli
AU - Nishibori, Masahiro
AU - Kawabata, Atsufumi
N1 - Funding Information:
This study was supported in part by Japan Society for the Promotion of Science , Grant-in-Aid for Scientific Research (C) 21K06608 (2021–2023) and 17K09046 (2017–2019) .
Publisher Copyright:
© 2021 The Authors
PY - 2022/1
Y1 - 2022/1
N2 - We examined the role of ATP and high mobility group box 1 (HMGB1) in paclitaxel-induced peripheral neuropathy (PIPN). PIPN in mice was prevented by HMGB1 neutralization, macrophage depletion, and P2X7 or P2X4 blockade. Paclitaxel and ATP synergistically released HMGB1 from macrophage-like RAW264.7 cells, but not neuron-like NG108-15 cells. The paclitaxel-induced HMGB1 release from RAW264.7 cells was accelerated by co-culture with NG108-15 cells in a manner dependent on P2X7 or P2X4. Paclitaxel released ATP from NG108-15 cells, but not RAW264.7 cells. Thus, PIPN is considered to involve acceleration of HMGB1 release from macrophages through P2X7 and P2X4 activation by neuron-derived ATP.
AB - We examined the role of ATP and high mobility group box 1 (HMGB1) in paclitaxel-induced peripheral neuropathy (PIPN). PIPN in mice was prevented by HMGB1 neutralization, macrophage depletion, and P2X7 or P2X4 blockade. Paclitaxel and ATP synergistically released HMGB1 from macrophage-like RAW264.7 cells, but not neuron-like NG108-15 cells. The paclitaxel-induced HMGB1 release from RAW264.7 cells was accelerated by co-culture with NG108-15 cells in a manner dependent on P2X7 or P2X4. Paclitaxel released ATP from NG108-15 cells, but not RAW264.7 cells. Thus, PIPN is considered to involve acceleration of HMGB1 release from macrophages through P2X7 and P2X4 activation by neuron-derived ATP.
KW - ATP
KW - Chemotherapy-induced peripheral neuropathy (CIPN)
KW - High mobility group box 1 (HMGB1)
KW - Neuroimmune crosstalk
KW - Paclitaxel
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U2 - 10.1016/j.jphs.2021.11.003
DO - 10.1016/j.jphs.2021.11.003
M3 - Article
C2 - 34924121
AN - SCOPUS:85119700783
SN - 1347-8613
VL - 148
SP - 156
EP - 161
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
IS - 1
ER -