TY - JOUR
T1 - Role of the hippocampal CA2 region following postischemic hypothermia in gerbil
AU - Yamashita, Shiro
AU - Miyamoto, Osamu
AU - Janjua, Najma A.
AU - Tomizawa, Kazuhito
AU - Matsui, Hideki
AU - Nakamura, Takehiro
AU - Nagao, Seigo
AU - Itano, Toshifumi
PY - 2003/3/17
Y1 - 2003/3/17
N2 - To investigate the changes in the principal subunit of N-methyl-D-aspartate (NMDA) receptor 1 (NR1) following the transient ischemia and postischemic hypothermia, in situ hybridization was used in the gerbil hippocampus. One of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors, Glutamate receptor 2 (GluR2) was also investigated to compare with NR1. Even at 1 day, NR1 and GluR2 mRNAs in the CA1 region were reduced following ischemia. Although postischemic hypothermia prevented almost all the neuronal cell death by ischemia and inhibited the reduction of NR1 and GluR2 mRNAs in the CA1 region after 7 days, the downregulation of NR1 mRNA in the CA2 region was observed even at 1 day. This change was specific for NR1 and not for GluR2. These results suggest that the changes in NR1 and GluR2 receptors at the mRNA level would occur in spite of postischemic hypothermia. The phenomenon in the CA2 region may play an important role to rescue neuronal cell death by ischemia.
AB - To investigate the changes in the principal subunit of N-methyl-D-aspartate (NMDA) receptor 1 (NR1) following the transient ischemia and postischemic hypothermia, in situ hybridization was used in the gerbil hippocampus. One of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors, Glutamate receptor 2 (GluR2) was also investigated to compare with NR1. Even at 1 day, NR1 and GluR2 mRNAs in the CA1 region were reduced following ischemia. Although postischemic hypothermia prevented almost all the neuronal cell death by ischemia and inhibited the reduction of NR1 and GluR2 mRNAs in the CA1 region after 7 days, the downregulation of NR1 mRNA in the CA2 region was observed even at 1 day. This change was specific for NR1 and not for GluR2. These results suggest that the changes in NR1 and GluR2 receptors at the mRNA level would occur in spite of postischemic hypothermia. The phenomenon in the CA2 region may play an important role to rescue neuronal cell death by ischemia.
KW - CA2 region
KW - Gerbils
KW - Hippocampus
KW - In situ hybridization
KW - NR1
KW - Postischemic hypothermia
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UR - http://www.scopus.com/inward/citedby.url?scp=0347926480&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(02)00664-2
DO - 10.1016/S0169-328X(02)00664-2
M3 - Article
C2 - 12654500
AN - SCOPUS:0347926480
SN - 0169-328X
VL - 111
SP - 8
EP - 16
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -