Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells

Teruko Nishio; Masato Maruyama; Toyokazu Yoshida; Yoshihiko Watanabe; Makiya Nishikawa; Yoshinobu Takakura

doi:10.1248/bpb.27.1653

Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK₁ cells

Teruko Nishio, Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe, Makiya Nishikawa, Yoshinobu Takakura

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK₁. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.

Original language	English
Pages (from-to)	1653-1655
Number of pages	3
Journal	Biological and Pharmaceutical Bulletin
Volume	27
Issue number	10
DOIs	https://doi.org/10.1248/bpb.27.1653
Publication status	Published - Oct 2004
Externally published	Yes

Keywords

Epithelial cell
Interferon
LLC-PK
Polarity
Secretory protein
μ1B

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Access to Document

10.1248/bpb.27.1653

Cite this

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title = "Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells",

abstract = "We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.",

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author = "Teruko Nishio and Masato Maruyama and Toyokazu Yoshida and Yoshihiko Watanabe and Makiya Nishikawa and Yoshinobu Takakura",

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AU - Nishio, Teruko

AU - Maruyama, Masato

AU - Yoshida, Toyokazu

AU - Watanabe, Yoshihiko

AU - Nishikawa, Makiya

AU - Takakura, Yoshinobu

PY - 2004/10

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