Selective augmenting effects of nitric oxide on antigen-specific IgE response in mice

Hitoshi Ohmori, Hiroshi Egusa, Norichika Ueura, Yuuki Matsumoto, Naoki Kanayama, Masaki Hikida

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Here, we report the enhancing effects of nitric oxide (NO) on an IgE antibody response in mice. Anti-trinitrophenyl (TNP) IgE production induced in vitro in TNP keyhole limpet hemocyanin (KLH)-primed spleen cells was inhibited by approximately 70% when an NO synthase (NOS) inhibitor, L-N(G)-monomethyl-L-arginine, was added at 10-7-10-6 M to the lymphocyte culture. On the other hand, addition of NO-generating agents to the culture resulted in a marked enhancement of the IgE production. In contrast, anti-TNP IgM and IgG1 responses were affected only marginally when the IgE production was either suppressed or augmented by these agents. NO did not directly augment IgE class switching in normal B cells stimulated with lipopolysaccharide and interleukin (IL)-4. NO-mediated augmentation of the IgE response is considered to be of a physiological significance because administration of aminoguanidine (AG), an inhibitor of inducible NOS, to immunized mice resulted in a preferential suppression of anti-TNP IgE production in vivo. This may be explained by the observation that AG-administration increased interferon-γ expression without changing that of IL-4 in the immunized mice. Taken together, these observations suggest a pathophysiological role of NO in the development of IgE-mediated allergic diseases. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
Issue number1
Publication statusPublished - Jan 2000


  • Allergy
  • IgE
  • Interferon-γ
  • Nitric oxide (NO)

ASJC Scopus subject areas

  • Pharmacology


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