Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death

A. Kohno; N. Emi; M. Kasai; M. Tanimoto; H. Saito

doi:10.1038/sj.gt.3300589

Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death

A. Kohno, N. Emi, M. Kasai, M. Tanimoto, H. Saito

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces non-structural proteins (nsPs) which replicate genomic RNA and amplify the mRNA encoding the structural proteins of SFV structural genes were replaced by a polylinker cassette to allow for insertion of heterologous DNA, was placed under the control of a cytomegalovirus immediate-early enhancer/promoter with a polyadenylation signal. Transfection of mammalian cells with this SFV-based plasmid vector, pSFV3-CMV-lacZ-pA, resulted in transient high-level expression of a β-glactosidase reporter gene.

Original language	English
Pages (from-to)	415-418
Number of pages	4
Journal	Gene Therapy
Volume	5
Issue number	3
DOIs	https://doi.org/10.1038/sj.gt.3300589
Publication status	Published - 1998

Keywords

Plasmid vector
Self-amplifying vector
Semliki Forest virus

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics

Access to Document

10.1038/sj.gt.3300589

Cite this

@article{da9700aab6ba4114b896396a820091de,

title = "Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death",

abstract = "We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces non-structural proteins (nsPs) which replicate genomic RNA and amplify the mRNA encoding the structural proteins of SFV structural genes were replaced by a polylinker cassette to allow for insertion of heterologous DNA, was placed under the control of a cytomegalovirus immediate-early enhancer/promoter with a polyadenylation signal. Transfection of mammalian cells with this SFV-based plasmid vector, pSFV3-CMV-lacZ-pA, resulted in transient high-level expression of a β-glactosidase reporter gene.",

keywords = "Plasmid vector, Self-amplifying vector, Semliki Forest virus",

author = "A. Kohno and N. Emi and M. Kasai and M. Tanimoto and H. Saito",

year = "1998",

doi = "10.1038/sj.gt.3300589",

language = "English",

volume = "5",

pages = "415--418",

journal = "Gene Therapy",

issn = "0969-7128",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Semliki Forest virus-based DNA expression vector

T2 - Transient protein production followed by cell death

AU - Kohno, A.

AU - Emi, N.

AU - Kasai, M.

AU - Tanimoto, M.

AU - Saito, H.

PY - 1998

Y1 - 1998

N2 - We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces non-structural proteins (nsPs) which replicate genomic RNA and amplify the mRNA encoding the structural proteins of SFV structural genes were replaced by a polylinker cassette to allow for insertion of heterologous DNA, was placed under the control of a cytomegalovirus immediate-early enhancer/promoter with a polyadenylation signal. Transfection of mammalian cells with this SFV-based plasmid vector, pSFV3-CMV-lacZ-pA, resulted in transient high-level expression of a β-glactosidase reporter gene.

AB - We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces non-structural proteins (nsPs) which replicate genomic RNA and amplify the mRNA encoding the structural proteins of SFV structural genes were replaced by a polylinker cassette to allow for insertion of heterologous DNA, was placed under the control of a cytomegalovirus immediate-early enhancer/promoter with a polyadenylation signal. Transfection of mammalian cells with this SFV-based plasmid vector, pSFV3-CMV-lacZ-pA, resulted in transient high-level expression of a β-glactosidase reporter gene.

KW - Plasmid vector

KW - Self-amplifying vector

KW - Semliki Forest virus

UR - http://www.scopus.com/inward/record.url?scp=0031885738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031885738&partnerID=8YFLogxK

U2 - 10.1038/sj.gt.3300589

DO - 10.1038/sj.gt.3300589

M3 - Article

C2 - 9614563

AN - SCOPUS:0031885738

SN - 0969-7128

VL - 5

SP - 415

EP - 418

JO - Gene Therapy

JF - Gene Therapy

IS - 3

ER -

Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this