TY - GEN
T1 - Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant
AU - Lorchirachoonkul, P.
AU - Yamashita, K.
AU - Noda, M.
AU - Shimanouchi, T.
AU - Umakoshi, H.
AU - Kuboi, R.
PY - 2010
Y1 - 2010
N2 - We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states and we can use this leakage current microsensor to detect other protein that do not have large interaction with the liposome.
AB - We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states and we can use this leakage current microsensor to detect other protein that do not have large interaction with the liposome.
UR - http://www.scopus.com/inward/record.url?scp=79951907486&partnerID=8YFLogxK
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U2 - 10.1109/ICSENS.2010.5690730
DO - 10.1109/ICSENS.2010.5690730
M3 - Conference contribution
AN - SCOPUS:79951907486
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 1056
EP - 1059
BT - IEEE Sensors 2010 Conference, SENSORS 2010
T2 - 9th IEEE Sensors Conference 2010, SENSORS 2010
Y2 - 1 November 2010 through 4 November 2010
ER -