TY - JOUR
T1 - Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant
AU - Noda, Minoru
AU - Lorchirachoonkul, Prinya
AU - Shimanouchi, Toshinori
AU - Yamashita, Kaoru
AU - Umakoshi, Hiroshi
AU - Kuboi, Ryouichi
N1 - Funding Information:
Manuscript received February 01, 2011; revised May 21, 2011; accepted May 24, 2011. Date of publication June 09, 2011; date of current version October 21, 2011. This work was supported in part by Grants-in-Aid for Scientific Research (Nos. 19360162, 21246121, 20760539) from the Japan Society for the Promotion of Science and in part by the Core University Program between JSPS and VAST. The associate editor coordinating the review of this paper and approving it for publication was Dr. Thomas Kenny.
PY - 2011
Y1 - 2011
N2 - We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect the existence of proteins. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully resulted in a prominent improvement in sensitivity by 129-folds of magnitude. This is because the modulation of conformation of the target proteins, carbonic anhydrase from bovine (CAB) and lysozyme could contribute to the promotion of the interaction between the liposome and the target protein.
AB - We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect the existence of proteins. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully resulted in a prominent improvement in sensitivity by 129-folds of magnitude. This is because the modulation of conformation of the target proteins, carbonic anhydrase from bovine (CAB) and lysozyme could contribute to the promotion of the interaction between the liposome and the target protein.
KW - Biochemical analysis
KW - bionanotechnology
KW - biosensors
KW - chemical and biological sensors
KW - liposome
KW - protein
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U2 - 10.1109/JSEN.2011.2159109
DO - 10.1109/JSEN.2011.2159109
M3 - Article
AN - SCOPUS:80054937551
SN - 1530-437X
VL - 11
SP - 2749
EP - 2755
JO - IEEE Sensors Journal
JF - IEEE Sensors Journal
IS - 11
M1 - 5871996
ER -