TY - JOUR
T1 - Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa
AU - Suzuki, Nobuhiro
AU - Watanabe, Yuzuko
AU - Kusano, Tomonobu
AU - Kitagawa, Yoshichika
N1 - Funding Information:
The authors are grateful to Dr. Hideaki ltoh for his very generous support In amino acid sequencing, Drs. Yukio Shirako, Kripamoy Aguan, Hiroshi Sano, and Thomas E. Bureau for their helpful advice, Ms. Mikiko Sugawara for preparation of the 114-kDa protein, Mr. Ma-sanobu Yoshikawa for helpful comments on protein electroelution, and Mr. Masahiro Yamanaka for supplying RDV-infected rice plants. We also thank Toshiyuki Takeshima for his kind assistance computer analysis and to Ms. Mayumi Shimizu for typing the manuscript. This work was partly supported by Research Grant 01760048 from the Ministry of Education, Japan.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1990/11
Y1 - 1990/11
N2 - The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.
AB - The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.
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U2 - 10.1016/0042-6822(90)90314-H
DO - 10.1016/0042-6822(90)90314-H
M3 - Article
C2 - 2219733
AN - SCOPUS:0025036274
SN - 0042-6822
VL - 179
SP - 455
EP - 459
JO - Virology
JF - Virology
IS - 1
ER -