SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Naoyoshi Kumakura; Atsushi Takeda; Yoichiro Fujioka; Hiroyasu Motose; Ryo Takano; Yuichiro Watanabe

doi:10.1016/j.febslet.2009.03.055

SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Naoyoshi Kumakura, Atsushi Takeda, Yoichiro Fujioka, Hiroyasu Motose, Ryo Takano, Yuichiro Watanabe

Research output: Contribution to journal › Article › peer-review

133 Citations (Scopus)

Abstract

Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. Structured summary: MINT-7014710: SGS3 (uniprotkb:Q9LDX1) and RDR6 (uniprotkb:Q9SG02) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809). MINT-7014697: RDR6 (uniprotkb:Q9SG02) and SGS3 (uniprotkb:Q9LDX1) colocalize (MI:0403) by fluorescence microscopy (MI:0416).

Original language	English
Pages (from-to)	1261-1266
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2009.03.055
Publication status	Published - Apr 17 2009
Externally published	Yes

Keywords

Arabidopsis
Dicer-like 4
Processing body
RNA silencing
RNA-dependent RNA polymerase 6
Suppressor of gene silencing 3

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2009.03.055

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title = "SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies",

abstract = "Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. Structured summary: MINT-7014710: SGS3 (uniprotkb:Q9LDX1) and RDR6 (uniprotkb:Q9SG02) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809). MINT-7014697: RDR6 (uniprotkb:Q9SG02) and SGS3 (uniprotkb:Q9LDX1) colocalize (MI:0403) by fluorescence microscopy (MI:0416).",

keywords = "Arabidopsis, Dicer-like 4, Processing body, RNA silencing, RNA-dependent RNA polymerase 6, Suppressor of gene silencing 3",

author = "Naoyoshi Kumakura and Atsushi Takeda and Yoichiro Fujioka and Hiroyasu Motose and Ryo Takano and Yuichiro Watanabe",

year = "2009",

month = apr,

day = "17",

doi = "10.1016/j.febslet.2009.03.055",

language = "English",

volume = "583",

pages = "1261--1266",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

AU - Kumakura, Naoyoshi

AU - Takeda, Atsushi

AU - Fujioka, Yoichiro

AU - Motose, Hiroyasu

AU - Takano, Ryo

AU - Watanabe, Yuichiro

PY - 2009/4/17

Y1 - 2009/4/17

N2 - Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. Structured summary: MINT-7014710: SGS3 (uniprotkb:Q9LDX1) and RDR6 (uniprotkb:Q9SG02) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809). MINT-7014697: RDR6 (uniprotkb:Q9SG02) and SGS3 (uniprotkb:Q9LDX1) colocalize (MI:0403) by fluorescence microscopy (MI:0416).

AB - Suppressor of gene silencing 3 (SGS3) is involved in RNA-dependent RNA polymerase 6 (RDR6)-dependent small-interfering RNA (siRNA) pathways in Arabidopsis. However, the roles of SGS3 in those pathways are unclear. Here, we show that SGS3 interacts and colocalizes with RDR6 in cytoplasmic granules. Interestingly, the granules containing SGS3 and RDR6 (named SGS3/RDR6-bodies) were distinct from the processing bodies where mRNAs are decayed and/or stored. Microscopic analyses and complementation experiments using SGS3-deletion mutants suggested that proper localization of SGS3 is important for its function. These results provide novel insights into RDR6-dependent siRNA formation in plants. Structured summary: MINT-7014710: SGS3 (uniprotkb:Q9LDX1) and RDR6 (uniprotkb:Q9SG02) physically interact (MI:0218) by bimolecular fluorescence complementation (MI:0809). MINT-7014697: RDR6 (uniprotkb:Q9SG02) and SGS3 (uniprotkb:Q9LDX1) colocalize (MI:0403) by fluorescence microscopy (MI:0416).

KW - Arabidopsis

KW - Dicer-like 4

KW - Processing body

KW - RNA silencing

KW - RNA-dependent RNA polymerase 6

KW - Suppressor of gene silencing 3

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ER -

SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies

Abstract

Keywords

ASJC Scopus subject areas

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