TY - JOUR
T1 - Short communication
T2 - Distribution of Porphyromonas gulae fimA genotypes in oral specimens from dogs with mitral regurgitation
AU - Shirai, Mitsuyuki
AU - Nomura, Ryota
AU - Kato, Yukio
AU - Murakami, Masaru
AU - Kondo, Chihiro
AU - Takahashi, Soraaki
AU - Yamasaki, Yoshie
AU - Matsumoto-Nakano, Michiyo
AU - Arai, Nobuaki
AU - Yasuda, Hidemi
AU - Nakano, Kazuhiko
AU - Asai, Fumitoshi
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research for Challenging Exploratory Research (No. 23658256 and 25670873 ) from the Japan Society for Promotion of Science , the Promotion and Mutual Aid Corporation of Private Schools of Japan , and a Grant-in-Aid for Matching Fund Subsidy for Private Universities. We also thank Prof. Howard K. Kuramitsu (State University of New York at Buffalo) for editing the manuscript.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Porphyromonas gulae, a suspected pathogen for periodontal disease in dogs, possesses approximately 41-kDa fimbriae (FimA) that are encoded by the fimA gene. In the present study, the association of fimA genotypes with mitral regurgitation (MR) was investigated. Twenty-five dogs diagnosed with MR (age range 6-13 years old, average 10.8 years) and 32 healthy dogs (8-15 years old, average 10.8 years) were selected at the participating clinics in a consecutive manner during the same time period. Oral swab specimens were collected from the dogs and bacterial DNA was extracted, then polymerase chain reaction analysis was performed using primers specific for each fimA genotype, with the dominant genotype determined. The rate for genotype C dominant specimens was 48.0% in the MR group, which was significantly higher than that in the control group (18.8%) (P < 0.05). These results suggest that P gulae fimA genotype C is associated with MR.
AB - Porphyromonas gulae, a suspected pathogen for periodontal disease in dogs, possesses approximately 41-kDa fimbriae (FimA) that are encoded by the fimA gene. In the present study, the association of fimA genotypes with mitral regurgitation (MR) was investigated. Twenty-five dogs diagnosed with MR (age range 6-13 years old, average 10.8 years) and 32 healthy dogs (8-15 years old, average 10.8 years) were selected at the participating clinics in a consecutive manner during the same time period. Oral swab specimens were collected from the dogs and bacterial DNA was extracted, then polymerase chain reaction analysis was performed using primers specific for each fimA genotype, with the dominant genotype determined. The rate for genotype C dominant specimens was 48.0% in the MR group, which was significantly higher than that in the control group (18.8%) (P < 0.05). These results suggest that P gulae fimA genotype C is associated with MR.
KW - Fimbriae
KW - Mitral regurgitation
KW - Periodontal disease
KW - Porphyromonas gulae
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U2 - 10.1016/j.rvsc.2015.07.009
DO - 10.1016/j.rvsc.2015.07.009
M3 - Article
C2 - 26412519
AN - SCOPUS:84942109578
SN - 0034-5288
VL - 102
SP - 49
EP - 52
JO - Research in Veterinary Science
JF - Research in Veterinary Science
ER -